Document Detail


Light and ultrastructural study of sciatic nerve lesions induced using intraneural injection of viable Mycobacterium leprae in normal and immunosuppressed Swiss white mice.
MedLine Citation:
PMID:  12120037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Freshly harvested M. leprae were microinjected into the sciatic nerves of nonimmunosuppressed (non-TR) and immunosuppressed (TR) mice using the technique described by Wisniewski and Bloom. The lesions thus induced, on bypassing the blood-nerve barrier, were biopsied at regular intervals beginning 24 hr and followed up to one year. The fate of M. leprae and the ensuing inflammation and nerve damage were studied using light and electron microscopy. The lesions in both non-TR and TR mice at 24 hr showed an influx of polymorphonuclear leukocytes and an increase in mast cells. The influx and peaking of lymphocytes were delayed by two weeks and 6 weeks, respectively, in TR mice, but the density of lymphocytes at the peak intervals was comparable in both. The plasma cells denoting the humoral response were seen in both, but there was a delay of 3 weeks in non-TR mice. The lesions in non-TR mice showed differentiation of macrophages into epithelioid cells and the formation of giant cells depicting borderline tuberculoid leprosy (BT), Whereas in TR mice, the macrophages showed foamy cytoplasmic changes depicting borderline lepromatous leprosy (BL). Other significant observations common to both non-TR and TR mice were: a) The lesions remained highly localized and showed signs of regression at the 6th and the 12th month intervals. b) The characteristic segmental demyelination and some attempt at remyelination were seen at the site. c) The influx of lymphocytes concorded well with demyelination. d) Bacteria were only seen in the macrophages and never in the Schwann cells or endothelial cells. e) Bacteria persisted in the macrophages, but appeared progressively degenerate at the 6th and 12th post-inoculation months, suggesting loss of viability. The study shows that there was a very effective containment of the infection and that the Schwann cells were resistant to M. leprae infection in the neural milieu. Nerve damage and Schwann cell bacillation do not go hand-in-hand.
Authors:
Vanaja Prabhakar Shetty; Noshir Hormusji Antia
Related Documents :
14571457 - Acetylcholine receptor distribution and synapse elimination at the developing neuromusc...
14631387 - Deficiency of the complement regulator cd59a enhances disease severity, demyelination a...
20091777 - Bdnf+/- mice exhibit deficits in oligodendrocyte lineage cells of the basal forebrain.
22005837 - Abnormal expression of muc5b in cftr-null mice and in mammary tumors of mmtv-ras mice.
9160427 - Accumulation of leucocytes and cytokines in the lactating ovine udder during mastitis d...
23669137 - Adolescent fluoxetine treatment decreases the effects of neonatal immune activation on ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association     Volume:  70     ISSN:  0148-916X     ISO Abbreviation:  Int. J. Lepr. Other Mycobact. Dis.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-07-17     Completed Date:  2002-07-31     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8505819     Medline TA:  Int J Lepr Other Mycobact Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  25-33     Citation Subset:  IM    
Affiliation:
Foundation for Medical Research, 84-A R.G. Thadani Marg, Worli, Bombay 400 018, India. frchbom@bom2.vsnl.net.in
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Female
Humans
Immunosuppression
Leprosy / microbiology,  pathology*,  physiopathology
Male
Mice
Microscopy, Electron
Mycobacterium leprae / pathogenicity*,  physiology
Sciatic Nerve / microbiology*,  physiopathology,  ultrastructure*
Thymectomy
Whole-Body Irradiation

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The paper grip test for screening on intrinsic muscle paralysis in the foot of leprosy patients.
Next Document:  Intraocular lens implantation in leprosy.