| Light sheet microscopy in cell biology. | |
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MedLine Citation:
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PMID: 23027001 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching, and good optical penetration depth. This unique combination of capabilities makes light sheet-based microscopes highly suitable for live imaging applications.Here, we provide an overview of light sheet-based microscopy assays for in vitro and in vivo imaging of biological samples, including cell extracts, soft gels, and large multicellular organisms. We furthermore describe computational tools for basic image processing and data inspection. |
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Authors:
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Raju Tomer; Khaled Khairy; Philipp J Keller |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 931 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-10-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 123-37 Citation Subset: IM |
Affiliation:
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Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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