| Light sheet fluorescence microscopy: a review. | |
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MedLine Citation:
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PMID: 21339178 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light, specimen photobleaching and phototoxicity are minimized compared to wide-field fluorescence, confocal, or multiphoton microscopy. LSFMs produce well-registered serial sections that are suitable for three-dimensional reconstruction of tissue structures. Because of a lack of a commercial LSFM microscope, numerous versions of light sheet microscopes have been constructed by different investigators. This review describes development of the technology, reviews existing devices, provides details of one LSFM device, and shows examples of images and three-dimensional reconstructions of tissues that were produced by LSFM. |
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Authors:
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Peter A Santi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 59 ISSN: 1551-5044 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-02-22 Completed Date: 2011-04-11 Revised Date: 2012-02-01 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 129-38 Citation Subset: IM |
Affiliation:
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Department of Otolaryngology, University of Minnesota, Minneapolis 55455, USA. psanti@umn.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Image Processing, Computer-Assisted Lasers Light* Microscopy, Fluorescence / instrumentation, methods* Specimen Handling |
| Grant Support | |
ID/Acronym/Agency:
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R01DC007588/DC/NIDCD NIH HHS; R01DC007588-03S1/DC/NIDCD NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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