Document Detail

Light scattering from nucleated biological cells.
MedLine Citation:
PMID:  1122336     Owner:  NLM     Status:  MEDLINE    
The light scattered from nucleated biological cells has been investigated by using four different theoretical models: an opaque disk, a homogeneous sphere, an opaque ring, and a coated sphere. By comparing these four models, diffraction at the edges of the cell and the nucleus has been found to be the predominate scattering mechanism for nucleated biological cells at low angles. The scattering patterns of nucleated cells are found to have a fine lobe (high-frequency) structure dependent on whole cell size, and an envelope lobe (low-frequency) structure dependent on relative nucleus size. The models indicate that the present technique for measuring cell size with a single low-angle light detector is highly dependent on the nucleus to cell diameter ratio. Whole cell size is better estimated by the ratio of the outputs from two low-angle detectors.
R A Meyer; A Brunsting
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  15     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  1975 Mar 
Date Detail:
Created Date:  1975-06-29     Completed Date:  1975-06-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  191-203     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus / ultrastructure*
Cells / ultrastructure*
Models, Biological
Scattering, Radiation

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