Document Detail

Light regulation of circadian clock-controlled gene expression in rice.
MedLine Citation:
PMID:  11489174     Owner:  NLM     Status:  MEDLINE    
Using transgenic rice seedlings expressing a firefly luciferase (luc) gene under the control of a rice CAB (chlorophyll a/b binding protein) promoter, we demonstrated how light affects circadian clocks in the transcription of the CAB gene. Rhythmic luc expression was observed for more than 5 d under constant light and dark (DD) conditions after light/dark entrainment. After a light pulse was applied at different time points in DD various temporal patterns of CAB gene expression were individually observed. We first examined two distinct properties related to the entrainment mechanism of the circadian clock: fluence-rate dependence of free-running periods (FRPs) and phase resetting by a light pulse. Although fluence-rate dependent shortening of FRP was demonstrated, the FRP in DD was almost equal to that in constant light of a middle fluence-rate, indicating that this fluence-rate dependence may not fully describe the entrainment of the circadian clock in rice. Typical phase responses of the circadian clock by a single light pulse were also observed at the transcriptional level in rice seedlings. Thus, the phase resettings upon the light/dark transitions of daily cycles may be sufficient to explain the entrainment mechanisms of rice. We have further demonstrated that, in addition to having a gating effect to acute response, a light pulse can activate the circadian clock-controlled CAB1R gene expression at the first circadian peak in a phase-dependent manner. This suggests that light activates circadian clock activity in the diurnal CAB gene expression under daily light/dark cycles.
N Sugiyama; T Izawa; T Oikawa; K Shimamoto
Related Documents :
10575044 - The hamster circadian rhythm system includes nuclei of the subcortical visual shell.
23536444 - Evidence for hearing loss in amblyopsid cavefishes.
9488654 - A screen for genes induced in the suprachiasmatic nucleus by light.
23039444 - Auditory filter tuning inferred with short sinusoidal and notched-noise maskers.
16665694 - Promotion of stomatal opening by indoleacetic acid and ethrel in epidermal strips of vi...
12654524 - Responses of the estrous cycle in dairy cows exposed to electric and magnetic fields (6...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  26     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-08-07     Completed Date:  2001-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  607-15     Citation Subset:  IM    
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/D12629;  X13908
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Circadian Rhythm / genetics*
Gene Expression Regulation, Plant / radiation effects*
Light-Harvesting Protein Complexes*
Luciferases / genetics
Molecular Sequence Data
Oryza sativa / genetics*
Photosynthetic Reaction Center Complex Proteins / genetics*
RNA, Messenger / genetics
Reg. No./Substance:
0/Light-Harvesting Protein Complexes; 0/Photosynthetic Reaction Center Complex Proteins; 0/RNA, Messenger; 0/fucoxanthin-, chlorophyll a-c-containing protein; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Involvement of a novel Arabidopsis phospholipase D, AtPLDdelta, in dehydration-inducible accumulatio...
Next Document:  Gene-associated CpG islands in plants as revealed by analyses of genomic sequences.