Document Detail


Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor.
MedLine Citation:
PMID:  22773814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rhizobium leguminosarum is a soil bacterium that infects root hairs and induces the formation of nitrogen-fixing nodules on leguminous plants. Light, oxygen, and voltage (LOV)-domain proteins are blue-light receptors found in higher plants and many algae, fungi, and bacteria. The genome of R. leguminosarum bv. viciae 3841, a pea-nodulating endosymbiont, encodes a sensor histidine kinase containing a LOV domain at the N-terminal end (R-LOV-HK). R-LOV-HK has a typical LOV domain absorption spectrum with broad bands in the blue and UV-A regions and shows a truncated photocycle. Here we show that the R-LOV-HK protein regulates attachment to an abiotic surface and production of flagellar proteins and exopolysaccharide in response to light. Also, illumination of bacterial cultures before inoculation of pea roots increases the number of nodules per plant and the number of intranodular bacteroids. The effects of light on nodulation are dependent on a functional lov gene. The results presented in this work suggest that light, sensed by R-LOV-HK, is an important environmental factor that controls adaptive responses and the symbiotic efficiency of R. leguminosarum.
Authors:
Hernán R Bonomi; Diana M Posadas; Gastón Paris; Mariela del Carmen Carrica; Marcus Frederickson; Lía Isabel Pietrasanta; Roberto A Bogomolni; Angeles Zorreguieta; Fernando A Goldbaum
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-05
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-25     Completed Date:  2012-10-12     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12135-40     Citation Subset:  IM    
Affiliation:
Fundación Instituto Leloir, IIBBA-Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina, Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Bacterial Adhesion / physiology*,  radiation effects
Base Sequence
Biofilms / growth & development
Blotting, Western
Flagella / metabolism
Gentian Violet
Light*
Microscopy, Electron, Scanning
Molecular Sequence Data
Peas / microbiology*
Photoreceptors, Microbial / metabolism*
Plant Root Nodulation / physiology*,  radiation effects
Polysaccharides, Bacterial / metabolism
Protein Kinases / genetics,  metabolism
Protein Structure, Tertiary / genetics
Real-Time Polymerase Chain Reaction
Rhizobium leguminosarum / physiology*,  radiation effects,  ultrastructure
Sequence Alignment
Sequence Analysis, DNA
Statistics, Nonparametric
Symbiosis*
Grant Support
ID/Acronym/Agency:
//Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/EPSI polysaccharide; 0/Photoreceptors, Microbial; 0/Polysaccharides, Bacterial; 548-62-9/Gentian Violet; EC 2.7.-/Protein Kinases; EC 2.7.3.-/protein-histidine kinase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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