Document Detail

Light quenching and depolarization of fluorescence observed with laser pulses. A new experimental opportunity in time-resolved fluorescence spectroscopy.
MedLine Citation:
PMID:  8018819     Owner:  NLM     Status:  MEDLINE    
We report the first time-resolved studies of quenching of fluorescence by light, i.e., "light quenching". The dye 4-(dicyanomethylene)-2-methyl-6-(p-dimethamino)-4H-pyrane (DCM) was excited in the anti-Stokes region from 560-600 nm. At high illumination power the intensities of DCM were sub-linear with incident power. The extent of light quenching was proportional to the emission spectrum at the incident wavelength, as expected for light-stimulated decay from the excited state. The frequency-domain intensity decays indicated the effect was not due to heating or other photochemical effects. Importantly, the decay time was unchanged, as expected for light quenching with a single pulsed laser beam, while the time-zero anisotropy was decreased due to orientation-dependent quenching of the excited state population. Light quenching of fluorescence provides a new method to control the excited state population and orientation of fluorophores, and offers new experimental opportunities for biophysical applications of time-resolved fluorescence.
I Gryczyński; V Bogdanov; J R Lakowicz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical chemistry     Volume:  49     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-08-04     Completed Date:  1994-08-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  223-32     Citation Subset:  IM    
Department of Biological Chemistry, University of Maryland at Baltimore, School of Medicine 21201.
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MeSH Terms
Fluorescent Dyes
Spectrometry, Fluorescence*
Grant Support
Reg. No./Substance:
0/Fluorescent Dyes

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