Document Detail

Light quality treatments enhance somatic seedling production in three southern pine species.
MedLine Citation:
PMID:  16356915     Owner:  NLM     Status:  MEDLINE    
Embryogenic cultures of loblolly pine (Pinus taeda L.), slash pine (Pinus elliottii Engelm.), longleaf pine (Pinus palustris Mill.) and slash pine x longleaf pine hybrids were initiated from immature seeds on an initiation medium containing 13.57 microM 2,4-dichlorophenoxyacetic acid and 2.22 microM benzylaminopurine. Embryogenic cultures proliferated and somatic embryos developed, matured and germinated following a modified protocol and media originally developed for radiata pine (Pinus radiata D. Don.) somatic seedling production. A discrete, light-sensitive pre-germination stage and a later germination (radicle emergence) stage were identified by the differential response of somatic embryos to light of different wavelengths. Different light quality treatments were applied during the pre-germination and germination steps, using cool white fluorescent bulbs or light-emitting diodes (LEDs), or both. In general, red wavelengths provided by LEDs during these steps resulted in higher frequencies of somatic embryo germination (up to 64%) and conversion (up to 50%), longer tap roots and more first-order lateral roots than the standard cool white fluorescent treatments or treatment with blue wavelengths from LEDs. In addition, exposure to red light allowed germination of somatic embryos of some clones that failed to produce germinants under fluorescent light. Germination and conversion were further enhanced by sequential application of cool white fluorescent light and red light, resulting in up to 100% germination and conversion in one experiment. Longleaf pine somatic embryos were especially responsive to the light quality treatments, resulting in the first report of somatic seedling production for this species.
Scott A Merkle; Paul M Montello; Xiuqin Xia; Bruce L Upchurch; Dale R Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tree physiology     Volume:  26     ISSN:  0829-318X     ISO Abbreviation:  Tree Physiol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2005-12-16     Completed Date:  2007-04-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955338     Medline TA:  Tree Physiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  187-94     Citation Subset:  IM    
Daniel B. Warnell School of Forest Resources, University of Georgia, Athens, GA 30602, USA.
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MeSH Terms
2,4-Dichlorophenoxyacetic Acid / pharmacology
Benzyl Compounds / pharmacology
Germination / drug effects,  radiation effects
Pinus / drug effects,  embryology,  radiation effects*
Pinus taeda / drug effects,  embryology,  radiation effects
Plant Growth Regulators / pharmacology
Plant Roots / drug effects,  growth & development,  radiation effects
Plant Stems / drug effects,  growth & development,  radiation effects
Purines / pharmacology
Seedling / drug effects,  embryology,  radiation effects*
Seeds / drug effects,  growth & development,  radiation effects*
Reg. No./Substance:
0/Benzyl Compounds; 0/Plant Growth Regulators; 0/Purines; 0/benzylaminopurine; 94-75-7/2,4-Dichlorophenoxyacetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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