| Light and oxygen are not required for harpin-induced cell death. | |
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MedLine Citation:
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PMID: 17951254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nicotiana sylvestris leaves challenged by the bacterial elicitor harpin N(Ea) were used as a model system in which to determine the respective roles of light, oxygen, photosynthesis, and respiration in the programmed cell death response in plants. The appearance of cell death markers, such as membrane damage, nuclear fragmentation, and induction of the stress-responsive element Tnt1, was observed in all conditions. However, the cell death process was delayed in the dark compared with the light, despite a similar accumulation of superoxide and hydrogen peroxide in the chloroplasts. In contrast, harpin-induced cell death was accelerated under very low oxygen (<0.1% O(2)) compared with air. Oxygen deprivation impaired accumulation of chloroplastic reactive oxygen species (ROS) and the induction of cytosolic antioxidant genes in both the light and the dark. It also attenuates the collapse of photosynthetic capacity and the respiratory burst driven by mitochondrial alternative oxidase activity observed in air. Since alternative oxidase is known to limit overreduction of the respiratory chain, these results strongly suggest that mitochondrial ROS accumulate in leaves elicited under low oxygen. We conclude that the harpin-induced cell death does not require ROS accumulation in the apoplast or in the chloroplasts but that mitochondrial ROS could be important in the orchestration of the cell suicide program. |
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Authors:
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Marie Garmier; Pierrick Priault; Guillaume Vidal; Simon Driscoll; Reda Djebbar; Martine Boccara; Chantal Mathieu; Christine H Foyer; Rosine De Paepe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-19 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 282 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-24 Completed Date: 2008-03-04 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 37556-66 Citation Subset: IM |
Affiliation:
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Institut de Biotechnologie des Plantes, Université Paris-Sud 11, UMR-CNRS 8618, Bâtiment 630, 91405, Orsay Cedex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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chemistry Carbon Dioxide / chemistry Cell Death Cell Nucleus / metabolism Electrolytes Hydrogen Peroxide / chemistry Light Models, Biological Oxygen / chemistry*, metabolism Photosynthesis Plant Leaves / metabolism Plant Physiological Phenomena Reactive Oxygen Species Superoxides / chemistry Tobacco / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Electrolytes; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 124-38-9/Carbon Dioxide; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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