Document Detail


Light microscopic immunolocalization of type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin in the basement membranes of a variety of rat organs.
MedLine Citation:
PMID:  6346854     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunohistochemical methods were used to determine whether type IV collagen, laminin, fibronectin, and heparan sulfate proteoglycan were present in diverse basement membranes. Antisera or antibodies against each substance were prepared, tested by enzyme-linked immunosorbent assay, and exposed to frozen sections of duodenum, trachea, kidney, spinal cord, cerebrum, and incisor tooth from rats aged 20 days to 34 months. Bound antibodies were then localized by indirect or direct peroxidase methods for examination in the light microscope. Immunostaining for type IV collagen, laminin, fibronectin, and heparan sulfate proteoglycan was observed in all of the basement membranes encountered. Fibronectin was also found in connective tissue. In general, the intensity of immunostaining was strong for type IV collagen and laminin, moderate for heparan sulfate proteoglycan, and weak for fibronectin. The pattern was similar in the age groups under study. Very recently the sulfated glycoprotein, entactin, was also detected in the basement membranes of the listed tissues in 20-day-old rats. It is accordingly proposed that, at least in the organs examined, type IV collagen, laminin, fibronectin, heparan sulfate proteoglycan, and entactin are present together in basement membranes.
Authors:
G W Laurie; C P Leblond; G R Martin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of anatomy     Volume:  167     ISSN:  0002-9106     ISO Abbreviation:  Am. J. Anat.     Publication Date:  1983 May 
Date Detail:
Created Date:  1983-08-11     Completed Date:  1983-08-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376312     Medline TA:  Am J Anat     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  71-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Basement Membrane / analysis*
Collagen / analysis*
Duodenum / analysis
Fibronectins / analysis*
Glycoproteins / analysis*
Heparin / analogs & derivatives*,  analysis
Immunoenzyme Techniques
Incisor / analysis
Kidney / analysis
Laminin
Proteoglycans / analysis*
Rats / metabolism*
Rats, Inbred Strains
Spinal Cord / analysis
Trachea / analysis
Grant Support
ID/Acronym/Agency:
1R01 DE 05690-01/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Fibronectins; 0/Glycoproteins; 0/Laminin; 0/Proteoglycans; 0/heparin proteoglycan; 9005-49-6/Heparin; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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