| Light-mediated TIM degradation within Drosophila pacemaker neurons (s-LNvs) is neither necessary nor sufficient for delay zone phase shifts. | |
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MedLine Citation:
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PMID: 20471351 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Circadian systems are entrained and phase shifted by light. In Drosophila, the model of light-mediated phase shifting begins with photon capture by CRYPTOCHROME (CRY) followed by rapid TIMELESS (TIM) degradation. In this study, we focused on phase delays and assayed TIM degradation within individual brain clock neurons in response to light pulses in the early night. Surprisingly, there was no detectable change in TIM staining intensity within the eight pacemaker s-LNvs. This indicates that TIM degradation within s-LNvs is not necessary for phase delays, and similar assays in other genotypes indicate that it is also not sufficient. In contrast, more dorsal circadian neurons appear light-sensitive in the early night. Because CRY is still necessary within the s-LNvs for phase shifting, the results challenge the canonical cell-autonomous molecular model and raise the question of how the pacemaker neuron transcription-translation clock is reset by light in the early night. |
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Authors:
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Chih-Hang Anthony Tang; Erica Hinteregger; Yuhua Shang; Michael Rosbash |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuron Volume: 66 ISSN: 1097-4199 ISO Abbreviation: Neuron Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-17 Completed Date: 2010-06-02 Revised Date: 2011-07-20 |
Medline Journal Info:
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Nlm Unique ID: 8809320 Medline TA: Neuron Country: United States |
Other Details:
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Languages: eng Pagination: 378-85 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Howard Hughes Medical Institute, National Center for Behavioral Genomics and Department of Biology, Brandeis University, Waltham, MA 02454, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Clocks / physiology Circadian Rhythm / physiology* Drosophila Drosophila Proteins / metabolism* F-Box Proteins / metabolism Fluorescent Antibody Technique Light Microscopy, Fluorescence Neurons / metabolism* Photoperiod Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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P01 NS044232-08/NS/NINDS NIH HHS; P01 NS44232/NS/NINDS NIH HHS; P30 NS045713-07/NS/NINDS NIH HHS; P30 NS45713/NS/NINDS NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/Drosophila Proteins; 0/F-Box Proteins; 0/JETLAG protein, Drosophila; 0/timeless protein, Drosophila |
| Comments/Corrections | |
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