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Light intensity and temperature affect systemic spread of silencing signal in transient agro-infiltration studies.
MedLine Citation:
PMID:  25220764     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
RNA silencing is a sequence-specific post-transcriptional gene inactivation mechanism that operates in diverse organisms and which can extend beyond its site of initiation, owing to the movement of the silencing signal, called non-autonomous gene silencing. Previous studies have shown that several factors manifest the movement of silencing signal, such as, the size (21 or 24 nt) of secondary siRNA produced, or the steady-state concentration of siRNAs and their cognate mRNA, or as a result of change in the sink-source status of plant parts affecting the phloem translocation. Our studies show that, both light intensity and temperature have significant impact on systemic movement of silencing signal in transient agro-infiltration studies in Nicotiana benthamiana. At higher light intensities (≥450 μEm(-2) s(-1) ) and higher temperatures (≥30°C), the gene silencing was localized to the leaf tissue that was infiltrated, without any systemic spread. Interestingly in these light and temperature conditions (≥450 μEm(-2) s(-1) and ≥30°C) the N. benthamiana plants showed recovery from the viral symptoms. However reduced systemic silencing and the reduced viral symptom severity at higher light intensities were due to the change in the sink-source status of the plant, ultimately affecting the phloem translocation of small RNAs or the viral genome. Whereas at lower light intensities (<300 μEm(-2) s(-1) ) with a constant temperature of 25°C, there was strong systemic movement of silencing signal in the N. benthamiana plants and also there was reduced recovery from virus infections. Accumulation of gene specific siRNAs were reduced at higher temperature as a result of reduction in the accumulation of transcript on transient agro-infiltration of RNAi constructs, mostly because of poor T-DNA transfer activity of Agrobacterium, possibly also accompanied by reduced phloem translocation.
Authors:
Basavaprabhu L Patil; Claude M Fauquet
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-14
Journal Detail:
Title:  Molecular plant pathology     Volume:  -     ISSN:  1364-3703     ISO Abbreviation:  Mol. Plant Pathol.     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-9-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954969     Medline TA:  Mol Plant Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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This article is protected by copyright. All rights reserved.
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