Document Detail

Light-induced pH changes in sub-bacterial particles of Halobacterium halobium. Effects of ionophores.
MedLine Citation:
PMID:  83878     Owner:  NLM     Status:  MEDLINE    
The kinetics of light-induced acidification and of the subsequent dark-induced alkalization in suspensions of sub-bacterial particles of Halobacterium halobium may be expressed as the sum of two exponentials, indicating two processes (Eisenbach, M., Bakker, E.P., Korenstein, R. and Caplan, S.R. (1976) FEBS Lett. 71, 228--232). We studied the effects of carbonyl cyanide p-trifluoromethyoxy phenyl-hydrazone, nigericin, gramicidin D, valinomycin, and monactin on the extents and the rate constants of the two processes. The various ionophores affected the two processes differently and in general the slower process was more sensitive to their presence. Valinomycin and monactin had relatively minor effects, apparently due to the high ionic strength of the suspension. When an artificial membrane potential was created in the dark, the light-induced acidification was preceded by a transient alkalization as is usually observed in intact cells. These results are discussed in the light of a suggested model accounting for the two processes (Caplan, S.R., Eisenbach, M., Cooper, S., Garty, H., Klemperer, G. and Bakker, E.P. (1977) in Bioenergetics of Membranes (Packer, L., Papageorgiou, G.C. and Trebst, A., eds.), pp. 101--114, Elsevier/North-Holland Biomedical Press, Amsterdam), taking into account the different selectivities of the ionophores applied.
H Garty; M Eisenbach; R Shuldman; S R Caplan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  545     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1979 Feb 
Date Detail:
Created Date:  1979-04-25     Completed Date:  1979-04-25     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  365-75     Citation Subset:  IM    
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MeSH Terms
Anti-Bacterial Agents / pharmacology
Bacteriorhodopsins / metabolism*
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / pharmacology
Carotenoids / metabolism*
Gramicidin / pharmacology
Halobacterium / drug effects*,  metabolism,  physiology
Hydrogen-Ion Concentration
Ionophores / pharmacology*
Lipid Metabolism*
Liposomes / metabolism
Membrane Potentials
Nigericin / pharmacology
Proteolipids / metabolism
Subcellular Fractions / drug effects*,  metabolism
Valinomycin / pharmacology
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Ionophores; 0/Liposomes; 0/Proteolipids; 1405-97-6/Gramicidin; 2001-95-8/Valinomycin; 28380-24-7/Nigericin; 36-88-4/Carotenoids; 370-86-5/Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; 53026-44-1/Bacteriorhodopsins

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