Document Detail


Light-induced blockage of cell division with a chromatin-targeted phototoxic fluorescent protein.
MedLine Citation:
PMID:  21214518     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Proteins of the Green Fluorescent Protein family are widely used as passive reporters for live cell imaging. Here we used tandem phototoxic fluorescent protein KillerRed fused to core histone H2B (H2B-tKR) as an active tool to affect cell division with light. We demonstrated that H2B-tKR-expressing cells behave normally in the dark, but transiently cease proliferation following green light illumination. Complete light-induced blockage of cell division for about 24 h was observed in cultured mammalian cells that were either transiently or stably transfected with H2B-tKR. Then illuminated cells restored normal division rate. X-ray cross complementing factor 1 (XRCC1) showed immediate redistribution in illuminated nuclei of H2B-tKR-expressing cells indicating massive light-induced damage of genomic DNA. Notably, nondisjunction of chromosomes was observed for cells illuminated during metaphase. In transgenic Xenopus embryos expressing H2B-tKR under control of tissue-specific promoters we observed clear retardation of development of corresponding tissues in green light illumined tadpoles. We believe that H2B-tKR represents a novel optogenetic tool, which can be used to study mitosis and meiosis progression per se, as well as to investigate the roles of specific cell populations in development, regeneration, and carcinogenesis in vivo.
Authors:
Ekaterina O Serebrovskaya; Tatiana V Gorodnicheva; Galina V Ermakova; Elena A Solovieva; George V Sharonov; Elena V Zagaynova; Dmitriy Mihaylovich Chudakov; Sergey Lukyanov; Andrey G Zaraisky; Konstantin A Lukyanov
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-10
Journal Detail:
Title:  The Biochemical journal     Volume:  -     ISSN:  1470-8728     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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