| Light and electron microscopic studies of the effects of p-chloroamphetamine on the monoaminergic innervation of the rat spinal cord. | |
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MedLine Citation:
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PMID: 8027444 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A previous report has shown that small diameter serotoninergic (5-HT) axons innervating the forebrain are selectively eliminated by treatment with an amphetamine derivative, (+/-)p-chloroamphetamine (PCA; Mamounas et al., [1991] J. Comp. Neurol. 314:558-586). It is well known that the spinal cord is the target of numerous monoaminergic fibers of different types. We have previously shown that the dorsal and ventral horns and the intermediolateral cell column are innervated by numerous serotonin-, noradrenaline- and dopamine-containing axons, including both thin axons with small varicosities and beaded axons with large varicosities. In all these regions, the large majority of fine indolaminergic fibers do not establish synaptic contacts, contrasting with the beaded axons which mostly exhibit synapses. The present work was conducted to study the effect of PCA on the monoaminergic innervation of the adult rat spinal cord. Animals received two subcutaneous doses of PCA 24 hours apart and were perfused 3 weeks later. Immunocytochemistry was performed to detect 5-HT, noradrenergic and dopaminergic fibers by using light and electron microscopy. Light microscopy revealed that PCA treatment caused a marked and selective elimination of the fine 5-HT-immunoreactive fibers, mainly found in the dorsal horn, but spared all other larger axons. This selective effect on the dorsal horn innervating thin 5-HT fibers was confirmed with the electron microscope by calculating the synaptic incidence(s) of monoaminergic innervation. These results suggest that fine and beaded 5-HT axons correspond to two anatomically, biochemically and pharmacologically different types of fibers, which could arise from two subpopulations of brainstem neurons. In addition, this drug could be used to provide an experimental animal, devoid of 5-HT nonsynaptic fibers, thereby facilitating a study on the role of dorsal horn nonsynaptic system in pain modulation. |
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Authors:
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J L Ridet; M Geffard; A Privat |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of comparative neurology Volume: 343 ISSN: 0021-9967 ISO Abbreviation: J. Comp. Neurol. Publication Date: 1994 May |
Date Detail:
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Created Date: 1994-08-10 Completed Date: 1994-08-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0406041 Medline TA: J Comp Neurol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 281-96 Citation Subset: IM |
Affiliation:
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INSERM U. 336-EPHE, Université Montpellier II, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Behavior, Animal / drug effects Dopamine / metabolism* Immunohistochemistry Male Microscopy, Electron Norepinephrine / metabolism* Rats Rats, Sprague-Dawley Serotonin / metabolism* Spinal Cord / drug effects*, metabolism* p-Chloroamphetamine / pharmacology* |
| Chemical | |
Reg. No./Substance:
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50-67-9/Serotonin; 51-41-2/Norepinephrine; 64-12-0/p-Chloroamphetamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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