Document Detail

Light-directed synthesis of peptide nucleic acids (PNAs) chips.
MedLine Citation:
PMID:  17236754     Owner:  NLM     Status:  MEDLINE    
We report herein the light-directed synthesis of peptide nucleic acids (PNAs) microarray using PNA monomers protected by photolabile protecting groups and a maskless technique that uses a digital micromirror array system to form virtual masks. An ultraviolet image from the virtual mask was cast onto the active surface of a glass substrate, which was mounted in a flow cell reaction chamber connected to a peptide synthesizer. Light exposure was followed by automatic chemical coupling cycles and these steps were repeated with different virtual masks to grow the desired PNA probes in a selected pattern. In a preliminary experiment, an array of PNA probes with dimensions of 4.11 mm x 4.11 mm was generated on each slide. Each synthesis region in the final array measured 210 microm x 210 microm for a total of 256 sites. The center-to-center space was 260 microm. It was observed from the hybridization pattern of the fluorescently labeled oligonucleotide targets that the fluorescence intensities of the matched, and mismatched sequences showed substantial difference, demonstrating specificity in the identification of complementary sequences. This opens the way to exploit processes from the microelectronics industry for the fabrication of PNA microarrays with high densities.
Zheng-Chun Liu; Dong-Sik Shin; Mohammadreza Shokouhimehr; Kook-Nyung Lee; Byung-Wook Yoo; Yong-Kweon Kim; Yoon-Sik Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-19
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  22     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-21     Completed Date:  2007-07-26     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  2891-7     Citation Subset:  IM    
School of Chemical and Biological Engineering, Seoul National University, Kwanak-Gu, Seoul 151-742, South Korea.
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MeSH Terms
Nucleic Acid Probes*
Oligonucleotide Array Sequence Analysis / methods*
Peptide Nucleic Acids*
Reg. No./Substance:
0/Nucleic Acid Probes; 0/Peptide Nucleic Acids

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