| Light-dependent hydration of the space surrounding photoreceptors in the cat retina. | |
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MedLine Citation:
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PMID: 7918224 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have studied the effect of retinal illumination on the concentration of the extracellular space marker tetramethylammonium (TMA+) in the dark-adapted cat retina using double-barreled ion-selective microelectrodes. The retina was loaded with TMA+ by a single intravitreal injection. Retinal illumination produced a slow decrease in [TMA+]o, which was maximal in amplitude in the most distal portion of the space surrounding photoreceptors, the subretinal space. The light-evoked decrease in [TMA+]o was considerably slower and of a different overall time course than the light-evoked decrease in [K+]o, also recorded in the subretinal space. [TMA+]o decreased to a peak at 38 s after the onset of illumination, then slowly recovered towards the baseline, and transiently increased following the offset of illumination. It resembled the light-evoked [TMA+]o decreases previously recorded in the in vitro preparations of frog (Huang & Karwoski, 1990, 1992) and chick (Li et al., 1992, 1994) but was considerably larger in amplitude, 22% compared with 7%. As in frog, where it was first recorded, the light-evoked [TMA+]o decrease is considered to originate from a light-evoked increase in the volume of the subretinal space (or subretinal hydration). A mathematical model accounting for [TMA+]o diffusion predicted that the volume increase underlying the response was 63% on average and could be as large as 95% and last for minutes. The estimated volume increase was then used to examine its effect on K+ concentration in the subretinal space. We conclude that a light-dependent hydration of the subretinal space represents a significant physiological event in the intact cat eye, which should affect the organization of the interphotoreceptor matrix, and the concentrations of all ions and metabolites located in the subretinal space. |
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Authors:
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J D Li; V I Govardovskii; R H Steinberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Visual neuroscience Volume: 11 ISSN: 0952-5238 ISO Abbreviation: Vis. Neurosci. Publication Date: 1994 Jul-Aug |
Date Detail:
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Created Date: 1994-10-27 Completed Date: 1994-10-27 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8809466 Medline TA: Vis Neurosci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 743-52 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of California, San Francisco 94143-0444. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Cats Dark Adaptation Electrophysiology Extracellular Space / metabolism* Ganglionic Stimulants / metabolism Ion-Selective Electrodes Photic Stimulation* Photoreceptor Cells / metabolism* Potassium / metabolism Quaternary Ammonium Compounds / metabolism Retina / metabolism, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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EY01429/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ganglionic Stimulants; 0/Quaternary Ammonium Compounds; 51-92-3/tetramethylammonium; 7440-09-7/Potassium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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