Document Detail


Light controls phospholipase A2alpha and beta gene expression in Citrus sinensis.
MedLine Citation:
PMID:  20388744     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The low-molecular weight secretory phospholipase A2alpha (CssPLA2alpha) and beta (CsPLA2beta) cloned in this study exhibited diurnal rhythmicity in leaf tissue of Citrus sinensis. Only CssPLA2alpha displayed distinct diurnal patterns in fruit tissues. CssPLA2alpha and CsPLA2beta diurnal expression exhibited periods of approximately 24 h; CssPLA2alpha amplitude averaged 990-fold in the leaf blades from field-grown trees, whereas CsPLA2beta amplitude averaged 6.4-fold. Diurnal oscillation of CssPLA2alpha and CsPLA2beta gene expression in the growth chamber experiments was markedly dampened 24 h after transfer to continuous light or dark conditions. CssPLA2alpha and CsPLA2beta expressions were redundantly mediated by blue, green, red and red/far-red light, but blue light was a major factor affecting CssPLA2alpha and CsPLA2beta expression. Total and low molecular weight CsPLA2 enzyme activity closely followed diurnal changes in CssPLA2alpha transcript expression in leaf blades of seedlings treated with low intensity blue light (24 micromol m(-2) s(-1)). Compared with CssPLA2alpha basal expression, CsPLA2beta expression was at least 10-fold higher. Diurnal fluctuation and light regulation of PLA2 gene expression and enzyme activity in citrus leaf and fruit tissues suggests that accompanying diurnal changes in lipophilic second messengers participate in the regulation of physiological processes associated with phospholipase A2 action.
Authors:
Hui-Ling Liao; Jacqueline K Burns
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Publication Detail:
Type:  Journal Article     Date:  2010-04-13
Journal Detail:
Title:  Journal of experimental botany     Volume:  61     ISSN:  1460-2431     ISO Abbreviation:  J. Exp. Bot.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-31     Completed Date:  2010-08-17     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9882906     Medline TA:  J Exp Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  2469-78     Citation Subset:  IM    
Affiliation:
University of Florida, IFAS, Horticultural Sciences Department, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, Florida 33850-2299, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Citrus sinensis / chemistry,  enzymology*,  genetics,  radiation effects
Darkness
Gene Expression Regulation, Enzymologic / radiation effects*
Group IV Phospholipases A2 / chemistry,  genetics*,  metabolism
Light
Molecular Sequence Data
Plant Proteins / chemistry,  genetics*,  metabolism
Protein Isoforms / genetics,  metabolism
Sequence Alignment
Chemical
Reg. No./Substance:
0/Plant Proteins; 0/Protein Isoforms; EC 3.1.1.4/Group IV Phospholipases A2
Comments/Corrections

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