Document Detail


Light-controlled synthetic gene circuits.
MedLine Citation:
PMID:  22633822     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Highly complex synthetic gene circuits have been engineered in living organisms to develop systems with new biological properties. A precise trigger to activate or deactivate these complex systems is desired in order to tightly control different parts of a synthetic or natural network. Light represents an excellent tool to achieve this goal as it can be regulated in timing, location, intensity, and wavelength, which allows for precise spatiotemporal control over genetic circuits. Recently, light has been used as a trigger to control the biological function of small molecules, oligonucleotides, and proteins involved as parts in gene circuits. Light activation has enabled the construction of unique systems in living organisms such as band-pass filters and edge-detectors in bacterial cells. Additionally, light also allows for the regulation of intermediate steps of complex dynamic pathways in mammalian cells such as those involved in kinase networks. Herein we describe recent advancements in the area of light-controlled synthetic networks.
Authors:
Laura Gardner; Alexander Deiters
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review     Date:  2012-05-25
Journal Detail:
Title:  Current opinion in chemical biology     Volume:  16     ISSN:  1879-0402     ISO Abbreviation:  Curr Opin Chem Biol     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-21     Completed Date:  2012-12-20     Revised Date:  2013-08-14    
Medline Journal Info:
Nlm Unique ID:  9811312     Medline TA:  Curr Opin Chem Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  292-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
North Carolina State University, Department of Chemistry, Raleigh, NC 27695, United States.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Gene Regulatory Networks / drug effects,  radiation effects*
Genetic Engineering / methods*
Humans
Light*
Photochemical Processes
Grant Support
ID/Acronym/Agency:
R01 GM079114/GM/NIGMS NIH HHS; R01GM79114/GM/NIGMS NIH HHS
Comments/Corrections

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