Document Detail


Light-addressable electrodeposition of cell-encapsulated alginate hydrogels for a cellular microarray using a digital micromirror device.
MedLine Citation:
PMID:  22685500     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This paper describes a light-addressable electrolytic system used to perform an electrodeposition of calcium alginate hydrogels using a digital micromirror device (DMD). In this system, a patterned light illumination is projected onto a photoconductive substrate serving as a photo-anode to electrolytically produce protons, which can lead to a decreased pH gradient. The low pH generated at the anode can locally release calcium ions from insoluble calcium carbonate (CaCO(3)) to cause gelation of calcium alginate through sol-gel transition. By controlling the illumination pattern on the DMD, a light-addressable electrodeposition of calcium alginate hydrogels with different shapes and sizes, as well as multiplexed micropatterning was performed. The effects of the concentration of the alginate and CaCO(3) solutions on the dimensional resolution of alginate hydrogel formation were experimentally examined. A 3 × 3 array of cell-encapsulated alginate hydrogels was also successfully demonstrated through light-addressable electrodeposition. Our proposed method provides a programmable method for the spatiotemporally controllable assembly of cell populations into cellular microarrays and could have a wide range of biological applications in cell-based biosensing, toxicology, and drug discovery.
Authors:
Shih-Hao Huang; Hui-Jung Hsueh; Yeu-Long Jiang
Publication Detail:
Type:  Journal Article     Date:  2011-08-01
Journal Detail:
Title:  Biomicrofluidics     Volume:  5     ISSN:  1932-1058     ISO Abbreviation:  Biomicrofluidics     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2012-06-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101293825     Medline TA:  Biomicrofluidics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  34109-3410910     Citation Subset:  -    
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