| Light activation of phospholipase A2 in the photoreceptor of the crayfish (Procambarus clarkii). | |
| | |
MedLine Citation:
|
PMID: 10769925 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Retinal lipids of crayfish, kept at 4 degrees C under continuous darkness for 3 weeks, consisted mainly of phosphatidylcholine (PC) and phosphatidylethanolamine (PE); sphingomyelin (SM), phosphatidylinositol (PI) and phosphatidylserine (PS) were minor contributors. PI, involved in the phototransduction cascade, never reached greater concentrations than 7% of the total. High concentrations of polyunsaturated fatty acids (PUFA) such as 20:4n-6, 20:5n-3 and 22:6n-3 (DHA, docosahexaenoic acid) were present in PC, PE and PS, but scarce in SM and PI. In retinae of crayfish kept at 4 degrees C in darkness for 3 weeks and then exposed to white light (6 h; ca. 4,500 lx), SM and PS remained seemingly unaffected. PC, however, significantly decreased within 10 min to 65% of the initial value and 50% at 180 min. To study the reduction of PC, lipids of retinae suspended in physiological solution with/without phospholipase C (PLC) and phospholipase A2 (PLA2) inhibitors such as DMDA (= DEDA), manoalide, ET-18-OCH3, and U-73122 were measured. Only free fatty acids (FFA) of retinae with inhibitors of PLA2 like DMDA and manoalide decreased. Retinae irradiated by white light for 3 h displayed a significant reduction of PC, compared with those that had remained in continuous darkness. However, the PC of retinae with PLA2-inhibitors was not decreased by light. Our results provide evidence that not only photoreceptor cell PLC, but also PLA2 is activated by light. |
| | |
Authors:
|
T Kashiwagi; V B Meyer-Rochow; K Nishimura; E Eguchi |
Related Documents
:
|
8123595 - Epidermal growth factor induces the production of biologically distinguishable diglycer... 2495005 - Increase of phosphatidylinositol arachidonic acid incorporation induced by mepacrine. 3110145 - Acetylcholine stimulates selective liberation and re-esterification of arachidonate and... 9057095 - Involvement of phospholipase d activation in endothelin-1-induced release of arachidoni... 7627145 - Amino acid transport after transient global ischemia in rats: quantitative autoradiogra... 8691455 - 4-[4-[(2-hydroxybenzoyl)amino]phenyl]butyric acid as a novel oral delivery agent for re... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Acta neurobiologiae experimentalis Volume: 60 ISSN: 0065-1400 ISO Abbreviation: Acta Neurobiol Exp (Wars) Publication Date: 2000 |
Date Detail:
|
Created Date: 2000-05-18 Completed Date: 2000-05-18 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 1246675 Medline TA: Acta Neurobiol Exp (Wars) Country: POLAND |
Other Details:
|
Languages: eng Pagination: 9-16 Citation Subset: IM; S |
Affiliation:
|
Department of Virology, School of Medicine, Kurume University, Fukuoka, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Astacoidea Cell Membrane / enzymology Dark Adaptation / physiology Enzyme Activation / physiology Estrenes / pharmacology Fatty Acids, Nonesterified / metabolism Fatty Acids, Unsaturated / metabolism, pharmacology Phosphatidylcholines / metabolism Phosphodiesterase Inhibitors / pharmacology Phospholipases A / metabolism* Phospholipases A2 Phospholipid Ethers / pharmacology Photic Stimulation Photoreceptor Cells, Invertebrate / drug effects, enzymology* Pyrrolidinones / pharmacology Terpenes / pharmacology Vision, Ocular / physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Estrenes; 0/Fatty Acids, Nonesterified; 0/Fatty Acids, Unsaturated; 0/Phosphatidylcholines; 0/Phosphodiesterase Inhibitors; 0/Phospholipid Ethers; 0/Pyrrolidinones; 0/Terpenes; 112648-68-7/1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; 65492-82-2/edelfosine; 75088-80-1/manoalide; 89560-01-0/7,7-dimethyl-5,8-eicosadienoic acid; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of acute hepatic encephalopathy on [3H]dopamine release from rat cerebral cortex and striatum...
Next Document: Categorization of unilaterally presented emotional words: an ERP analysis.