Document Detail


Light dose is a limiting factor to maintain cell viability in fluorescence microscopy and single molecule detection.
MedLine Citation:
PMID:  20479994     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
A test system for cell viability based on colony formation has been established and applied to high resolution fluorescence microscopy and single molecule detection. Living cells were irradiated either by epi-illumination or by total internal reflection (TIR) of a laser beam, and light doses where at least 90% of irradiated cells survived were determined. These light doses were in the range of a few J/cm(2) up to about 200 J/cm(2) depending on the wavelength of illumination as well as on the presence or absence of a fluorescent dye (e.g., the membrane marker laurdan). In general, cells were less sensitive to TIR than to epi-illumination. However, comparably high light doses needed for repetitive excitation of single molecules limit the application of super-resolution microscopy to living cells.
Authors:
Michael Wagner; Petra Weber; Thomas Bruns; Wolfgang S L Strauss; Rainer Wittig; Herbert Schneckenburger
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Publication Detail:
Type:  Journal Article     Date:  2010-03-08
Journal Detail:
Title:  International journal of molecular sciences     Volume:  11     ISSN:  1422-0067     ISO Abbreviation:  Int J Mol Sci     Publication Date:  2010  
Date Detail:
Created Date:  2010-05-18     Completed Date:  2010-07-01     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101092791     Medline TA:  Int J Mol Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  956-66     Citation Subset:  -    
Affiliation:
Institut für Angewandte Forschung, Hochschule Aalen, Germany. michael.wagner@htw-aalen.de
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