| Light dose is a limiting factor to maintain cell viability in fluorescence microscopy and single molecule detection. | |
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MedLine Citation:
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PMID: 20479994 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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A test system for cell viability based on colony formation has been established and applied to high resolution fluorescence microscopy and single molecule detection. Living cells were irradiated either by epi-illumination or by total internal reflection (TIR) of a laser beam, and light doses where at least 90% of irradiated cells survived were determined. These light doses were in the range of a few J/cm(2) up to about 200 J/cm(2) depending on the wavelength of illumination as well as on the presence or absence of a fluorescent dye (e.g., the membrane marker laurdan). In general, cells were less sensitive to TIR than to epi-illumination. However, comparably high light doses needed for repetitive excitation of single molecules limit the application of super-resolution microscopy to living cells. |
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Authors:
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Michael Wagner; Petra Weber; Thomas Bruns; Wolfgang S L Strauss; Rainer Wittig; Herbert Schneckenburger |
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Publication Detail:
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Type: Journal Article Date: 2010-03-08 |
Journal Detail:
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Title: International journal of molecular sciences Volume: 11 ISSN: 1422-0067 ISO Abbreviation: Int J Mol Sci Publication Date: 2010 |
Date Detail:
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Created Date: 2010-05-18 Completed Date: 2010-07-01 Revised Date: 2013-05-29 |
Medline Journal Info:
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Nlm Unique ID: 101092791 Medline TA: Int J Mol Sci Country: Switzerland |
Other Details:
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Languages: eng Pagination: 956-66 Citation Subset: - |
Affiliation:
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Institut für Angewandte Forschung, Hochschule Aalen, Germany. michael.wagner@htw-aalen.de |
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