Document Detail


Ligation of the left circumflex coronary artery with subsequent MRI and histopathology in rabbits.
MedLine Citation:
PMID:  21205450     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Provided is the surgical procedure for ligating the left circumflex coronary artery to simulate heart ischemia by using a rabbit model. Heart rate monitored by electrocardiogram was increased at 5 min after ligation (mean ± SEM, 205 ± 13 bpm) when compared with that before ligation (170 ± 12 bpm), but returned to baseline at 25 min after ligation (183 ± 11 bpm). A marked elevation in the ST segment and reduction of the QRS wave of the electrocardiogram indicated the evolving myocardial infarct. The ejection fraction derived from MRI was decreased by 20% in the infarcted heart. The extent of necrosis and fibrosis in the myocardium due to ischemia led to decreased compliance and efficiency of the left ventricle. Masson trichrome staining showed blue-stained fibrils with the appearance of loose, threadlike scar tissue dispersed transmurally in the left ventricle and extending toward the apex. This study demonstrates the feasibility of MRI analysis of myocardial infarction in a rabbit model. The myocardial architecture, including the geometry of the myofibers which determines the contractile function of the heart, is clearly demonstrated by using cardiac MRI. Understanding the 3-dimensional arrangement of the myocardial microstructure and how remodeling of the infarcted myocardium affects cardiac function in an animal model has important implications for the study of heart disease in humans.
Authors:
Norman Hu; Catherine M Straub; Aida A Garzarelli; Kyle H Sabey; James W Yockman; David A Bull
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of the American Association for Laboratory Animal Science : JAALAS     Volume:  49     ISSN:  1559-6109     ISO Abbreviation:  J. Am. Assoc. Lab. Anim. Sci.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2011-01-05     Completed Date:  2011-08-03     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101269489     Medline TA:  J Am Assoc Lab Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  838-44     Citation Subset:  IM    
Affiliation:
Division of Cardiothoracic Surgery, Department of Surgery, University of Utah, Salt Lake City, Utah, USA. norm.hu@hsc.utah.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Coronary Vessels / surgery*
Electrocardiography / veterinary
Heart Rate
Heart Ventricles / pathology
Ligation / instrumentation,  methods,  veterinary*
Magnetic Resonance Imaging / veterinary
Male
Models, Animal
Myocardial Infarction / pathology,  veterinary*
Myocardium / pathology
Rabbits*
Staining and Labeling
Surgery, Veterinary / methods*
Thoracotomy / veterinary
Grant Support
ID/Acronym/Agency:
HL071541/HL/NHLBI NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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