Document Detail


Ligation of the WC1 receptor induces gamma delta T cell growth arrest through fumonisin B1-sensitive increases in cellular ceramide.
MedLine Citation:
PMID:  11034356     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ceramide is a powerful regulator of cell fate, inducing either apoptosis or growth arrest. We have previously shown that an Ab to the gammadelta T cell-specific orphan receptor, WC1, is able to induce growth arrest in proliferating IL-2-dependent gammadelta T cells. We now show that this WC1-mediated growth arrest is associated with an increase in cellular ceramide, in the absence of any measurable changes in acidic/neutral sphingomyelinase activity. Moreover, cell-permeable analogues of ceramide also mimicked WC1-induced growth arrest along with an associated decrease in pocket protein expression and phosphorylation status. An important role for ceramide in WC1-induced growth arrest was confirmed by demonstrating that the specific ceramide synthase inhibitor fumonisin B1 blocked WC1-induced growth arrest and the associated molecular effects on the pocket proteins. Finally, we observed constitutive expression of both antiapoptotic factors bcl-2 and bcl-X, the former having increased expression upon WC1 stimulation. It is therefore proposed that ligation of WC1 leads to an accumulation in cellular ceramide through activation of ceramide synthase. This in turn results in a decreased overall expression of the pocket proteins pRb and p107, their hypophosphorylation, and an eventual growth arrest of the gammadelta T cell. To our knowledge, these results demonstrate for the first time that cell surface receptor-mediated ceramide synthase activation can affect cell fate through increases in cellular ceramide and provide further evidence that the orphan receptor WC1 regulates gammadelta T cell biology through a novel signaling pathway.
Authors:
P A Kirkham; H H Takamatsu; E W Lam; R M Parkhouse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  165     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-16     Completed Date:  2000-11-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3564-70     Citation Subset:  AIM; IM    
Affiliation:
Department of Immunology, Institute for Animal Health, Pirbright, Surrey, United Kingdom. paul.kirkham@pharma.novartis.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / pharmacology
Apoptosis / immunology
Carboxylic Acids / pharmacology*
Cell Division / immunology
Cell Line
Ceramides / biosynthesis*,  metabolism,  physiology
Diglycerides / metabolism
Enzyme Inhibitors / pharmacology
Fumonisins*
Ligands
Lymphocyte Activation / immunology
Membrane Glycoproteins / antagonists & inhibitors,  immunology*,  metabolism*,  physiology
Mice
Mycotoxins / pharmacology
Oxidoreductases / antagonists & inhibitors
Proto-Oncogene Proteins c-bcl-2 / biosynthesis,  physiology
Receptors, Antigen, T-Cell, gamma-delta / physiology*
T-Lymphocyte Subsets / cytology*,  enzymology,  immunology*,  metabolism
bcl-X Protein
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Bcl2l1 protein, mouse; 0/Carboxylic Acids; 0/Ceramides; 0/DMBT1 protein, Bos taurus; 0/Diglycerides; 0/Enzyme Inhibitors; 0/Fumonisins; 0/Ligands; 0/Membrane Glycoproteins; 0/Mycotoxins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Receptors, Antigen, T-Cell, gamma-delta; 0/bcl-X Protein; 116355-83-0/fumonisin B1; EC 1.-/Oxidoreductases; EC 1.3.1.-/dihydroceramide desaturase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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