Document Detail


Ligand- and species-dependent activation of PPARalpha.
MedLine Citation:
PMID:  15319530     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peroxisome proliferator-activated receptor alpha (PPARalpha) is mainly expressed in liver and involved in lipid metabolism. Oxidation of certain fatty acids in peroxisomes is under PPARalpha control. A wide variety of lipid molecules activate PPARalpha as well as the fibric acid derivative clofibrate. In the present study, we evaluated the differential activation of PPARalpha with several agonist ligands through its expression and DNA binding in both rat (McA-RH7777) and human (HepG2) hepatoma cell lines. In McA-RH7777 cells, clofibrate alone mediated a higher induction of PPARalpha expression than linoleic acid. In contrast, linoleic acid was the most effective ligand in HepG2 cells and treatment with clofibrate plus linoleic acid did not further increase PPARalpha expression. PPRE-binding activity of PPARalpha in ligand-treated cells was also increased in a parallel manner. We suggest that ligand-induced PPARalpha activation might give rise to differential species-dependent responses.
Authors:
Filiz Akbiyik; Denise M Ray; Hakan Bozkaya; Ediz Demirpence
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  14     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2004  
Date Detail:
Created Date:  2004-08-20     Completed Date:  2005-02-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  269-76     Citation Subset:  IM    
Copyright Information:
Copyright 2004 S. Karger AG, Basel
Affiliation:
Hacettepe University Faculty of Medicine, Department of Biochemistry, Ankara, Turkey. fakbiyik@hacettepe.edu.tr
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Hepatocellular / metabolism
Cell Line, Tumor
Cell Survival
Clofibrate / pharmacology
DNA / metabolism
Electrophoretic Mobility Shift Assay
Fatty Acids, Unsaturated / metabolism,  pharmacology,  physiology
Humans
Ligands
Linoleic Acid / pharmacology,  physiology
Liver / metabolism
PPAR alpha / agonists*,  analysis,  physiology
Rats
Species Specificity
Grant Support
ID/Acronym/Agency:
DE 11390/DE/NIDCR NIH HHS; ES01247/ES/NIEHS NIH HHS; T32-DE07165/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Ligands; 0/PPAR alpha; 2197-37-7/Linoleic Acid; 637-07-0/Clofibrate; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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