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Ligand-induced changes in Oestrogen and thyroid hormone receptor expression in the developing rat cerebellum: A comparative quantitative PCR and Western blot study.
MedLine Citation:
PMID:  22609997     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Oestrogen (E2) and thyroid hormones (THs) are key regulators of cerebellar development. Recent reports implicate a complex mechanism through which E2 and THs influence the expression levels of each other's receptors (ERs and TRs) to precisely mediate developmental signals and modulate signal strength. We examined the modulating effects of E2 and THs on the expression levels of their receptor mRNAs and proteins in cultured cerebellar cells obtained from 7-day-old rat pups. Cerebellar granule cell cultures were treated with either E2, THs or a combination of these hormones, and resulting receptor expression levels were determined by quantitative PCR and Western blot techniques. The results were compared to non-treated controls and to samples obtained from 14-day-old in situ cerebella. Additionally, we determined the glial effects on the regulation of ER-TR expression levels. The results show that (i) ER and TR expression depends on the combined presence of E2 and THs; (ii) glial cells mediate the hormonal regulation of neuronal ER-TR expression and (iii) loss of tissue integrity results in characteristic changes in ER-TR expression levels. These observations suggest that both E2 and THs, in adequate amounts, are required for the precise orchestration of cerebellar development and that alterations in the ratio of E2/THs may influence signalling mechanisms involved in neurodevelopment. Comparison of data from in vitro and in situ samples revealed a shift in receptor expression levels after loss of tissue integrity, suggesting that such adjusting/regenerative mechanisms may function after cerebellar tissue injury as well.
Authors:
Trudy Johnson Scalise; Andrea Győrffy; István Tóth; Dávid Sándor Kiss; Virág Somogyi; Gréta Goszleth; Tibor Bartha; László V Frenyó; Attila Zsarnovszky
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta veterinaria Hungarica     Volume:  60     ISSN:  0236-6290     ISO Abbreviation:  Acta Vet. Hung.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406376     Medline TA:  Acta Vet Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  263-84     Citation Subset:  IM    
Affiliation:
Szent István University Department of Physiology and Biochemistry, Faculty of Veterinary Sciences István u. 2 H-1078 Budapest Hungary.
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