| Ligand-dependent transcriptional activities of four torafugu pufferfish Takifugu rubripes peroxisome proliferator-activated receptors. | |
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MedLine Citation:
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PMID: 17632107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Structural and functional properties were investigated for four peroxisome proliferator-activated receptors (PPARs), PPARalpha1, PPARalpha2, PPARbeta, and PPARgamma, from torafugu pufferfish Takifugu rubripes and determined for their transcriptional activity by the reporter assay using reporter plasmids containing three copies of the acyl-CoA oxidase PPAR response element. Although torafugu PPARs showed a high similarity in the primary structure to other vertebrate counterparts, torafugu PPARalpha2 and gamma contained additional sequences of 21 and 28 amino acids, respectively, as in the case of other teleost fish species when compared with African clawed frog counterparts. The transcriptional activity of torafugu PPARalpha1 was enhanced 4.5- and 11.5-fold by Wy-14643 and 5,8,11,14-eicosatetraynoic acid (ETYA) each at 10 microM, respectively, whereas that of PPARalpha2, 4.5- and 7.3-fold at the same concentration of the respective ligands, respectively. The activities of torafugu PPARalpha1 and alpha2 were also enhanced 5.6- and 6.3-fold by ETYA at 1 microM, respectively, but not by Wy-14643 at this concentration. Furthermore, the activities of the two torafugu PPARalphas were enhanced 4.3- and 7.6-fold by arachidonic acid, 4.4- and 5.2-fold by docosahexaenoic acid, and 6.7- and 8.0-fold by eicosapentaenoic acid each at 50 microM, respectively. On the other hand, the activities of torafugu PPARbeta and gamma were not changed by Wy-14643, ETYA, rosiglitazone, nor PUFAs. These results suggest that the activities of torafugu PPARbeta and gamma require undefined ligands. Alternatively, the molecular mechanisms involved in their activation are different from those of other vertebrates. |
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Authors:
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Hidehiro Kondo; Ryohei Misaki; Laurent Gelman; Shugo Watabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-12 |
Journal Detail:
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Title: General and comparative endocrinology Volume: 154 ISSN: 0016-6480 ISO Abbreviation: Gen. Comp. Endocrinol. Publication Date: 2007 Oct-Dec |
Date Detail:
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Created Date: 2007-09-10 Completed Date: 2007-12-13 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370735 Medline TA: Gen Comp Endocrinol Country: United States |
Other Details:
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Languages: eng Pagination: 120-7 Citation Subset: IM |
Affiliation:
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Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB275885; AB275888 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Cells, Cultured Ligands* Molecular Sequence Data Peroxisome Proliferator-Activated Receptors / metabolism* Sequence Homology, Amino Acid Takifugu / genetics*, metabolism Transcriptional Activation* / drug effects Transfection |
| Chemical | |
Reg. No./Substance:
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0/Ligands; 0/Peroxisome Proliferator-Activated Receptors |
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