| Life span prediction from the rate of age-related DNA demethylation in normal and cancer cell lines. | |
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MedLine Citation:
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PMID: 8557095 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method has been proposed for the Hayflick Limit prediction by the analysis of the 5-methylcytosine content in DNA at earlier and later cell passages. The following facts were used as the basis of the method: (i) the rate of m5C loss from DNA remains approximately constant during cell divisions and it does not depend on the cell donor age; (ii) this rate is inversely proportional to the Hayflick Limit as well as to the life span of cell donor species; (iii) the period corresponded to loss of all m5C residues from the genome coincides with or somewhat exceeds the Hayflick Limit of normal cells. The prognosis of the Hayflick Limit has usually been found in good agreement with the experimental evidences for various human, hamster, and mouse cell lines. The method proposed may be used for early detection of precrisis and cancer cells. The age-related m5C loss may result from accumulation of the m5C-->T+C transitions occurring with DNA methylation in every cell division. |
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Authors:
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A L Mazin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental gerontology Volume: 30 ISSN: 0531-5565 ISO Abbreviation: Exp. Gerontol. Publication Date: 1995 Sep-Oct |
Date Detail:
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Created Date: 1996-02-26 Completed Date: 1996-02-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 475-84 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Bases of Ontogenesis, A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov State University, Moscow. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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genetics*,
metabolism Animals Cell Division / genetics Cell Line Cricetinae Cytosine / metabolism DNA / metabolism* DNA (Cytosine-5-)-Methyltransferase DNA, Neoplasm / metabolism* Female Humans Life Expectancy* Methylation Mice Neoplasms / genetics* Reference Values Species Specificity Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 71-30-7/Cytosine; 9007-49-2/DNA; EC 2.1.1.37/DNA (Cytosine-5-)-Methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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