| Life cycle analysis of unicellular algae. | |
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MedLine Citation:
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PMID: 20373493 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Unicellular green alga is a very convenient object for flow cytometric characterization. Flow cytometry has been proposed as a quick and reliable tool for studying life cycle and growth of unicellular algae. Cell size of vegetating algae can be monitored in association with their DNA and endogenous chlorophyll content. Cells of interest (e.g., group of cells of a certain stage of the life cycle) in an asynchronously proliferating cell population can be sorted out for further microscopical or molecular biology studies. This methodological approach can be helpful for researchers who are interested in algal proliferation. |
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Authors:
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Bogdan I Gerashchenko; Toshiyuki Takahashi; Toshikazu Kosaka; Hiroshi Hosoya |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] Volume: Chapter 11 ISSN: 1934-9300 ISO Abbreviation: Curr Protoc Cytom Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-07 Completed Date: 2010-06-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100899351 Medline TA: Curr Protoc Cytom Country: United States |
Other Details:
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Languages: eng Pagination: Unit 11.19.1-6 Citation Subset: IM |
Copyright Information:
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(c) 2010 by John Wiley & Sons, Inc. |
Affiliation:
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RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, Ukraine. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Algae, Green
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metabolism,
physiology* Cell Culture Techniques Chlorophyll / chemistry Cytological Techniques DNA / metabolism DNA, Algal / metabolism Flow Cytometry / methods* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Algal; 1406-65-1/Chlorophyll; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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