| Liberation of desmosine and isodesmosine as amino acids from insoluble elastin by elastolytic proteases. | |
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MedLine Citation:
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PMID: 21726534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The development of atherosclerotic lesions and abdominal aortic aneurysms involves degradation and loss of extracellular matrix components, such as collagen and elastin. Releases of the elastin cross-links desmosine (DES) and isodesmosine (IDE) may reflect elastin degradation in cardiovascular diseases. This study investigated the production of soluble elastin cross-linking structures by proteinases implicated in arterial diseases. Recombinant MMP-12 and neutrophil elastase liberated DES and IDE as amino acids from insoluble elastin. DES and IDE were also released from insoluble elastin exposed to monocyte/macrophage cell lines or human primary macrophages derived from peripheral blood monocytes. Elastin oxidized by reactive oxygen species (ROS) liberated more unconjugated DES and IDE than did non-oxidized elastin when incubated with MMP-12 or neutrophil elastase. These results support the exploration of free DES and IDE as biomarkers of elastin degradation. |
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Authors:
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Hideyuki Umeda; Masanori Aikawa; Peter Libby |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-06-25 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 411 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-08-01 Completed Date: 2011-10-03 Revised Date: 2012-09-25 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 281-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / metabolism Cardiovascular Diseases / metabolism* Cell Line Desmosine / metabolism* Elastin / metabolism* Humans Isodesmosine / metabolism* Leukocyte Elastase / chemistry, genetics, metabolism* Matrix Metalloproteinase 12 / chemistry, genetics, metabolism* Mice Reactive Oxygen Species / metabolism Recombinant Proteins / chemistry, genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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HL080472/HL/NHLBI NIH HHS; R01 HL080472-07/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Reactive Oxygen Species; 0/Recombinant Proteins; 11003-57-9/Desmosine; 9007-58-3/Elastin; 991-01-5/Isodesmosine; EC 3.4.21.37/Leukocyte Elastase; EC 3.4.24.65/Matrix Metalloproteinase 12 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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