Document Detail


Liberation of desmosine and isodesmosine as amino acids from insoluble elastin by elastolytic proteases.
MedLine Citation:
PMID:  21726534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of atherosclerotic lesions and abdominal aortic aneurysms involves degradation and loss of extracellular matrix components, such as collagen and elastin. Releases of the elastin cross-links desmosine (DES) and isodesmosine (IDE) may reflect elastin degradation in cardiovascular diseases. This study investigated the production of soluble elastin cross-linking structures by proteinases implicated in arterial diseases. Recombinant MMP-12 and neutrophil elastase liberated DES and IDE as amino acids from insoluble elastin. DES and IDE were also released from insoluble elastin exposed to monocyte/macrophage cell lines or human primary macrophages derived from peripheral blood monocytes. Elastin oxidized by reactive oxygen species (ROS) liberated more unconjugated DES and IDE than did non-oxidized elastin when incubated with MMP-12 or neutrophil elastase. These results support the exploration of free DES and IDE as biomarkers of elastin degradation.
Authors:
Hideyuki Umeda; Masanori Aikawa; Peter Libby
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-06-25
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  411     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-08-01     Completed Date:  2011-10-03     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  281-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Cardiovascular Diseases / metabolism*
Cell Line
Desmosine / metabolism*
Elastin / metabolism*
Humans
Isodesmosine / metabolism*
Leukocyte Elastase / chemistry,  genetics,  metabolism*
Matrix Metalloproteinase 12 / chemistry,  genetics,  metabolism*
Mice
Reactive Oxygen Species / metabolism
Recombinant Proteins / chemistry,  genetics,  metabolism
Grant Support
ID/Acronym/Agency:
HL080472/HL/NHLBI NIH HHS; R01 HL080472-07/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Reactive Oxygen Species; 0/Recombinant Proteins; 11003-57-9/Desmosine; 9007-58-3/Elastin; 991-01-5/Isodesmosine; EC 3.4.21.37/Leukocyte Elastase; EC 3.4.24.65/Matrix Metalloproteinase 12
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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