Document Detail


Lgr5-EGFP marks taste bud stem/progenitor cells in posterior tongue.
MedLine Citation:
PMID:  23377989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Until recently, reliable markers for adult stem cells have been lacking for many regenerative mammalian tissues. Lgr5 (leucine-rich repeat-containing G-protein-coupled receptor 5) has been identified as a marker for adult stem cells in intestine, stomach, and hair follicle; Lgr5-expressing cells give rise to all types of cells in these tissues. Taste epithelium also regenerates constantly, yet the identity of adult taste stem cells remains elusive. In this study, we found that Lgr5 is strongly expressed in cells at the bottom of trench areas at the base of circumvallate (CV) and foliate taste papillae and weakly expressed in the basal area of taste buds and that Lgr5-expressing cells in posterior tongue are a subset of K14-positive epithelial cells. Lineage-tracing experiments using an inducible Cre knockin allele in combination with Rosa26-LacZ and Rosa26-tdTomato reporter strains showed that Lgr5-expressing cells gave rise to taste cells, perigemmal cells, along with self-renewing cells at the bottom of trench areas at the base of CV and foliate papillae. Moreover, using subtype-specific taste markers, we found that Lgr5-expressing cell progeny include all three major types of adult taste cells. Our results indicate that Lgr5 may mark adult taste stem or progenitor cells in the posterior portion of the tongue.
Authors:
Karen K Yee; Yan Li; Kevin M Redding; Ken Iwatsuki; Robert F Margolskee; Peihua Jiang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  31     ISSN:  1549-4918     ISO Abbreviation:  Stem Cells     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-25     Completed Date:  2014-05-02     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  992-1000     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 AlphaMed Press.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Lineage
Fluorescent Dyes / chemistry
Green Fluorescent Proteins / biosynthesis,  chemistry*,  genetics
Intestines / cytology,  metabolism
Male
Mice
Mice, Transgenic
Receptors, G-Protein-Coupled / biosynthesis*,  chemistry,  genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells / cytology*,  metabolism
Taste Buds / cytology*,  metabolism
Tongue / cytology*,  metabolism
Grant Support
ID/Acronym/Agency:
1P30DC011735-01/DC/NIDCD NIH HHS; DC003055/DC/NIDCD NIH HHS; DC0101842/DC/NIDCD NIH HHS; DK081421/DK/NIDDK NIH HHS; P30 DC011735/DC/NIDCD NIH HHS; R01 DC003055/DC/NIDCD NIH HHS; R01 DC010842/DC/NIDCD NIH HHS; R01 DK081421/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Lgr5 protein, mouse; 0/Receptors, G-Protein-Coupled; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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