| Lgr4-deficient mice showed premature differentiation of ureteric bud with reduced expression of Wnt effector Lef1 and Gata3. | |
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MedLine Citation:
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PMID: 21523854 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We previously reported that Lgr4 has a critical role in the morphogenesis of kidney, but the detailed functions of Lgr4 in kidney development have not been elucidated. In contrast to Lgr4 null mice with 129Ola × C57BL/6J mixed background, C57BL/6J-backcrossed Lgr4 null mice (Lgr4(-/-) ) showed the severe phenotype of embryonic lethality and also had dilated tubules in kidneys at E16.5. Based on quantitative RT-PCR and in situ hybridization, branching morphogenesis at E15.5 in the Lgr4(-/-) was arrested earlier, and both DBA-lectin staining and immunohistochemical analysis using Aqp3 antibodies showed that the ureteric bud (UB) of Lgr4(-/-) kidneys underwent premature differentiation. Furthermore, quantitative RT-PCR and histological analysis suggested that the impaired UB differentiation was caused by down-regulation of the Wnt pathway and Gata3 in the Lgr4(-/-) kidneys. We demonstrate here that Lgr4 has a novel function for maintaining the UB in an undifferentiated state. Developmental Dynamics, 2011. © 2011 Wiley-Liss, Inc. |
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Authors:
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Yasuaki Mohri; Kazunori Oyama; Atsushi Akamatsu; Shigeki Kato; Katsuhiko Nishimori |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-26 |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: - ISSN: 1097-0177 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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