Document Detail

LexA chimeras reveal the function of Drosophila Fos as a context-dependent transcriptional activator.
MedLine Citation:
PMID:  10805795     Owner:  NLM     Status:  MEDLINE    
The transcriptional activation potential of proteins can be assayed in chimeras containing a heterologous DNA-binding domain that mediates their recruitment to reporter genes. This approach has been widely used in yeast and in transient mammalian cell assays. Here, we applied it to assay the transactivation potential of proteins in transgenic Drosophila embryos. We found that a chimera between the DNA-binding bacterial LexA protein and the transactivation domain from yeast GAL4 behaved as a potent synthetic activator in all embryonic tissues. In contrast, a LexA chimera containing Drosophila Fos (Dfos) required an unexpected degree of context to function as a transcriptional activator. We provide evidence to suggest that this context is provided by Djun and Mad (a Drosophila Smad), and that these partner factors need to be activated by signaling from Jun N-terminal kinase and decapentaplegic, respectively. Because Dfos behaves as an autonomous transcriptional activator in more artificial assays systems, our data suggest that context-dependence of transcription factors may be more prevalent than previously thought.
D Szüts; M Bienz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  97     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-06-13     Completed Date:  2000-06-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5351-6     Citation Subset:  IM    
Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom.
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MeSH Terms
Animals, Genetically Modified
Bacterial Proteins / metabolism*
Base Sequence
DNA-Binding Proteins / genetics*
Drosophila Proteins*
Drosophila melanogaster / embryology,  genetics*
Embryo, Nonmammalian / physiology
Enhancer Elements, Genetic
Fungal Proteins / genetics,  metabolism
Homeodomain Proteins / genetics*
Insect Proteins / metabolism
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases / metabolism
Proto-Oncogene Proteins c-fos / metabolism*
Recombinant Fusion Proteins / metabolism
Repressor Proteins / metabolism
Saccharomyces cerevisiae Proteins*
Serine Endopeptidases / metabolism*
Signal Transduction
Transcription Factors / genetics,  metabolism
Transcriptional Activation
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Drosophila Proteins; 0/Fungal Proteins; 0/GAL4 protein, S cerevisiae; 0/Homeodomain Proteins; 0/Insect Proteins; 0/LexA protein, Bacteria; 0/Proto-Oncogene Proteins c-fos; 0/Recombinant Fusion Proteins; 0/Repressor Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; 0/Ubx protein, Drosophila; 0/dpp protein, Drosophila; EC Mitogen-Activated Protein Kinases; EC Protein Kinases; EC 3.4.21.-/Serine Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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