| Levocetrizine has anti-inflammatory effects against Toll-like receptor (TLR)3 through the inhibition of Toll-IL-1 receptor (TIR)-domain-containing adapter inducing IFN-beta (TRIF) and receptor-interacting protein (RIP). | |
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MedLine Citation:
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PMID: 21330073 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the anti-inflammatory effect of levocetrizine (LCEZ) on the intracellular adhesion molecule-1 (ICAM-1) in human nasal epithelial cells stimulated by TLR3 and further analyze the anti-inflammatory mechanism of LCEZ in the MyD88-independent pathway before NF-κB is activated. METHODS: A primary culture of human nasal epithelial cells (HNECs) was generated from nasal polyps. After stimulation of epithelial cells with LTA, double-stranded RNA (dsRNA), and LPS, reverse transcription-polymerase chain reaction (RT-PCR) was performed at 1, 6, and 24h to clarify the optimal stimulation of ICAM-1 in HNECs. To investigate the anti-inflammatory effects of LCEZ, HNECs were pretreated with three different concentrations of LCEZ (500, 50, and 5nM) for 2h. HNECs were washed and then stimulated with dsRNA. At 1, 6, and 24h after stimulation, the level of ICAM-1 was measured by RT-PCR and ELISA. Western blots for TRIF and RIP were performed. RESULTS: The level of ICAM-1 was significantly elevated by dsRNA. Pretreatment with LCEZ decreased the secretion of ICAM-1, which was observed in RT-PCR and Western blots but not in ELISA analyses. The expression of TRIF and RIP, measured by Western blot, was decreased by pretreatment with LCEZ. CONCLUSION: The activation of HNECs by TLRs (especially TLR3) could trigger an inflammatory process, which might be inhibited by LCEZ through the suppression of TRIF and RIP proteins. |
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Authors:
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Chan-Soon Park; Jin-Hee Cho; Jun-Myung Kang; Byung-Kook Kim; Min-Ah Han |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-15 |
Journal Detail:
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Title: Auris, nasus, larynx Volume: - ISSN: 1879-1476 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7708170 Medline TA: Auris Nasus Larynx Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Crown Copyright © 2011. Published by Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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