Document Detail

Levels of minimal residual disease detected by quantitative molecular monitoring herald relapse in patients with multiple myeloma.
MedLine Citation:
PMID:  15136219     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND OBJECTIVES:Detection of minimal residual disease (MRD) has helped to improve the treatment of patients with leukemia. At present MRD testing in patients with multiple myeloma (MM) is not applied as a standard diagnostic or prognostic method. DESIGN AND METHODS: Immunoglobulin heavy chain (IgH) polymerase chain reaction (PCR) using patient-specific TaqMan probes together with LightCycler technology was performed to quantify minimal residual disease in MM. Relative levels of clonotypic cells were assessed as IgH/2beta-actin ratios with a sensitivity of 10(-4) to 10(-5). RESULTS: Following stem cell transplantation, a significant reduction of clonotypic cells was observed in bone marrow (BM) and peripheral blood (PB) samples of 11 patients, comparing pre-treatment values with those of best response (median: 13% to 0.09% and 0.03% to 0%, respectively). In 5 patients with ongoing clinical remission IgH/2beta-actin ratios remained stable at a low level, while in 6 patients an increase to 2% in BM and 0.4% in PB was associated with progression of the disease. In 4 of these 6 patients the increase of clonotypic cells in PB was detectable a median of 3 months (range: 0.5-6) before relapse. Furthermore, time-to-progression of patients with pre-transplantation IgH/2b-actin ratios > 0.03% in BM was significantly shorter than that of patients with lower MRD levels. INTERPRETATION AND CONCLUSIONS: MRD in patients with MM can be quantified reliably using TaqMan chemistry adapted to the LightCycler system. Residual tumor cell levels before transplantation as well as results of sequential molecular monitoring are predictive of relapse.
Roland Fenk; Muharrem Ak; Guido Kobbe; Ulrich Steidl; Carolin Arnold; Mark Korthals; Ali Hünerlitürkoglu; Ulrich-Peter Rohr; Slawomir Kliszewski; Alf Bernhardt; Rainer Haas; Ralf Kronenwett
Related Documents :
1545569 - A pilot study of carboplatin augmentation therapy for patients with poor risk acute non...
6849839 - Coexistent myelodysplasia and plasma cell neoplasia.
8642049 - Clinical implications of cytogenetic classification in adult acute lymphoblastic leukae...
12673719 - Phase ii study of su5416--a small-molecule, vascular endothelial growth factor tyrosine...
8366299 - Usefulness of terminal deoxynucleotidyl transferase as prognosticator in leukemia patie...
2302659 - Fatal pulmonary failure complicating high-dose cytosine arabinoside therapy in acute le...
16037729 - Modeling adherence to therapeutic regimens in patients with hypertension.
3336259 - The role of needle aspiration biopsy in the selection of patients for thyroidectomy.
24642379 - Liver function, in-hospital and post-discharge clinical outcome in patients with acute ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Haematologica     Volume:  89     ISSN:  1592-8721     ISO Abbreviation:  Haematologica     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-11     Completed Date:  2006-04-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417435     Medline TA:  Haematologica     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  557-66     Citation Subset:  IM    
Dept. of Hematology, Oncology and Clinical Immunology, University of Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Actins / blood
Bone Marrow Cells / cytology
Cell Line, Tumor
Clone Cells / cytology
DNA Probes / blood,  diagnostic use*
Immunoglobulin Heavy Chains / blood
Middle Aged
Multiple Myeloma / blood,  complications*
Neoplasm, Residual / complications,  diagnosis*
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Stem Cell Transplantation
Reg. No./Substance:
0/Actins; 0/DNA Probes; 0/Immunoglobulin Heavy Chains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A low serum level of soluble tumor necrosis factor receptor p55 predicts response to thalidomide in ...
Next Document:  Sequential analysis of biochemical markers of bone resorption and bone densitometry in multiple myel...