Document Detail


Levels and enterotoxigenicity of Clostridium perfringens in pozole, tamales, and birria.
MedLine Citation:
PMID:  15726977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A quantitative survey of Clostridium perfringens in typical foods served at local restaurants was conducted for 18 months in Guadalajara, Mexico. A total of 151 samples, including goat's birria (50), pozole (50), and beef tamales (51), were collected from small restaurants in Guadalajara. Samples were tested for C. perfringens by the most probable number (MPN) method and for mesophilic aerobic plate counts (MAPCs) and coliform, yeast, and mold counts by plate count methods. Isolates confirmed as C. perfringens were further sporulated and tested for cytotoxic or cytotonic effect against Vero cells as an indication of enterotoxin production. C. perfringens was detected in 78 (52%) of all samples at concentrations that ranged from 2.3 to 5.4 log MPN/g. Average MAPCs were 1.3 to 2.7 log CFU/g, depending on the type of dish. Coliform counts ranged from less than 1.0 to 1.5 CFU/g, and yeast and mold counts were less than 1.0 log CFU/g in all cases. A total of 118 isolates of C. perfringens were tested for enterotoxic effect on Vero cells; 82 (70%) showed activity against Vero cells. Of them, 31 isolates induced cell lysis, indicating cytotoxic effect; 41 induced cell elongation, indicating cytotonic effect; and 10 produced both cytotoxic and cytotonic effect. Dilution of the bacterial filtrates that were still producing an effect on Vero cells ranged from 1:80 to 1:5,120. These results underscore the importance of determining enterotoxigenicity when testing for C. perfringens in foods.
Authors:
V Navarro-Hidalgo; E Cabrera-Díaz; H Zepeda; L Mota de la Garza; A Castillo; R Torres-Vitela
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  68     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-24     Completed Date:  2005-03-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  331-5     Citation Subset:  IM    
Affiliation:
Laboratorio de Microbiología Sanitaria, Centro Universitario de Ciencias Exactas e Ingeniería, Universidad de Guadalajara, Marcelino Garcia Barragán 1451, Guadalajara 44430, Mexico.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cercopithecus aethiops
Clostridium perfringens / isolation & purification*,  metabolism,  pathogenicity
Colony Count, Microbial
Consumer Product Safety*
Enterotoxins / biosynthesis
Food Contamination / analysis*
Food Handling / methods*
Food Microbiology
Goats
Humans
Meat Products / microbiology*
Mexico
Temperature
Vero Cells
Chemical
Reg. No./Substance:
0/Enterotoxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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