Document Detail


Levels of corticotrophin-releasing hormone receptor subtype 1 mRNA in pregnancy and during labour in human myometrium measured by quantitative competitive PCR.
MedLine Citation:
PMID:  9801463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is suggested that corticotrophin-releasing hormone (CRH) is involved in parturition. We have previously reported the presence of the CRH receptor subtype 1 (CRH R1) in human uterine myocytes. The aim of the present study was to investigate whether expression of the CRH R1 in myometrial tissue changes in pregnancy and labour. We used a quantitative competitive PCR method to measure the mRNA levels of this receptor in non-pregnant and in term pregnant myometrium before and at different stages of labour. The levels of mRNA for the housekeeping gene for glucocerebrosidase (GCB) were also determined. The results were expressed as a ratio of CRH R1 and GCB mRNA levels. We have found that in pregnancy the CRH R1 is down-regulated from a ratio of 0.093+/-0.011 in non-pregnant myometrium to 0.012+/-0.005 (P<0.001) in term non-labouring myometrium. No significant changes were observed in the CRH R1:GCB ratio in tissues sampled within 13 h (0.013+/-0.004) from the start of labour. In summary, normalised levels of CRH R1 are down-regulated in pregnancy and do not change during labour. We speculate that our results do not support a direct role for the CRH R1 receptor in myometrial stimulation.
Authors:
B Rodríguez-Liñares; S Phaneuf; A López Bernal; E A Linton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular endocrinology     Volume:  21     ISSN:  0952-5041     ISO Abbreviation:  J. Mol. Endocrinol.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-01-07     Completed Date:  1999-01-07     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  8902617     Medline TA:  J Mol Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  201-8     Citation Subset:  IM    
Affiliation:
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Women's Centre, John Radcliffe Hospital, UK.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Female
Humans
Labor, Obstetric*
Mice
Myometrium / metabolism*
Pregnancy*
RNA, Messenger / genetics,  metabolism*
Receptors, Corticotropin-Releasing Hormone / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, Corticotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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