Document Detail


Levels of cathepsins S and H in pleural fluids of inflammatory and neoplastic origin.
MedLine Citation:
PMID:  19404922     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cathepsins S and H are present in immune cells and tissues and may play a role in the activation of an adoptive immune response. Our goal was to assess their protein levels in pleural fluids from 82 patients who underwent thoracentesis or thoracoscopy for therapeutic or diagnostic reasons and to relate them to an inflammatory, neoplastic or hemodynamic origin. Pleural effusions were also analyzed for a panel of 13 inflammatory or proliferative markers to test possible links to a nonspecific host reaction. Increased levels of cathepsin S were found in parainflammatory and cancer-related effusions compared to transudates. Cathepsin H levels were elevated only in parainflammatory effusions, whereas the levels in cancer-related effusions were comparable to transudates. Cathepsin S values significantly correlated with LDH, alpha-1-AT, VEGF, sICAM, sVCAM, MPO, uPA, MMP-9/TIMP-1, IL-8 and MCP-1, but not with CRP, IL-10 or cathepsin H. In contrast to cathepsin S, cathepsin H values did not correlate with markers of inflammation, indicating a specific role for cathepsin H in the pleural host response. In conclusion, the estimation of cathepsin S and cathepsin H may help to distinguish between effusions of different etiology.
Authors:
Karin Bunatova; Natasa Obermajer; Jaromir Kotyza; Milos Pesek; Janko Kos
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of biological markers     Volume:  24     ISSN:  0393-6155     ISO Abbreviation:  Int. J. Biol. Markers     Publication Date:    2009 Jan-Mar
Date Detail:
Created Date:  2009-04-30     Completed Date:  2009-08-12     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8712411     Medline TA:  Int J Biol Markers     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  47-51     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Medical Faculty, Charles University, Plzen, Czech Republic. karin.bunatova@lfp.cuni.cz
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MeSH Terms
Descriptor/Qualifier:
Aged
Biological Markers / blood,  metabolism
Cathepsin H
Cathepsins / blood,  metabolism*
Cysteine Endopeptidases / blood,  metabolism*
Female
Humans
Inflammation / enzymology
Lung Neoplasms / enzymology
Male
Middle Aged
Paraneoplastic Syndromes / enzymology
Pleural Effusion / enzymology*
Pleural Effusion, Malignant / enzymology*
Pleurisy / enzymology
Tumor Markers, Biological / blood,  metabolism*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Tumor Markers, Biological; EC 3.4.-/Cathepsins; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.16/CTSH protein, human; EC 3.4.22.16/Cathepsin H; EC 3.4.22.27/cathepsin S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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