Document Detail

Level of HOXA5 hypermethylation in acute myeloid leukemia is associated with short-term outcome.
MedLine Citation:
PMID:  20890077     Owner:  NLM     Status:  MEDLINE    
Hypermethylation of the homeobox (HOX) gene promoter leads to decreased expression of the gene during tumor development and is thought to be correlated with the clinical outcome in leukemia. In this study, we performed pyrosequencing to quantify the methylation level of HOXA5 genes in the bone marrow samples obtained from 50 patients with AML and 19 normal controls. The methylation percentage of HOXA5 in AML patients (median=65.4%, interquartile range=35.9-72.3%) was higher than that of HOXA5 in control patients (median=43.1%, interquartile range=36.7-49.6%, Mann-Whitney U test, P=0.012). The patients of the AML group who had a high methylation percentage (>70%) had a good prognosis with a 3-yr overall survival (OS) of 82.5%, whereas the patients with a low methylation percentage (≤70%) showed a 3-yr OS of 40.5% (P=0.048). Cox proportional hazards regression showed that the methylation percentages of HOXA5 were independently associated with the 3-yr OS of AML patients, regardless of their karyotypes. We propose that the quantification of HOXA5 methylation by pyrosequencing may be useful for predicting short-term prognosis in AML. However, the limitations of our study are the small sample size and its preliminary nature. Thus, a larger study should be performed to clearly determine the relationships among HOXA5 methylation levels, cytogenetics, and prognosis in AML patients.
Shine Young Kim; Sang-Hyun Hwang; Eun Joo Song; Ho Jin Shin; Joo Seop Jung; Eun Yup Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Korean journal of laboratory medicine     Volume:  30     ISSN:  1598-6535     ISO Abbreviation:  Korean J Lab Med     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2011-02-17     Revised Date:  2014-05-30    
Medline Journal Info:
Nlm Unique ID:  101322822     Medline TA:  Korean J Lab Med     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  469-73     Citation Subset:  IM    
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MeSH Terms
DNA Methylation*
Homeodomain Proteins / genetics*
Leukemia, Myeloid, Acute / genetics,  mortality*
Middle Aged
Promoter Regions, Genetic
ROC Curve
Sequence Analysis, DNA
Survival Rate
Reg. No./Substance:
0/HOXA5 protein, human; 0/Homeodomain Proteins

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