Document Detail

Leukotriene induction of TxB2 in cultured bovine aortic endothelial cells.
MedLine Citation:
PMID:  6092275     Owner:  NLM     Status:  MEDLINE    
The leukotrienes (LT) are potent mediators of inflammation, capable of inducing plasma leakage from postcapillary venules and vasoconstriction of terminal arterioles. Some microvascular effects may be attributable to LT stimulation of thromboxane (Tx) synthesis. Incubation of primary cultures of bovine aortic endothelial cells with buffer, LTB4 (10(-8) M) or LTD4 (10(-8)M), resulted in TxA2 production in pg/10(5) cells to the extent of: 67 +/- 20, 571 +/- 180, and 333 +/- 60 respectively, as measured by radioimmunoassay of the stable metabolite TxB2. Endothelial pretreatment with the LTD4 receptor antagonist FPL55712 (10(-5)M) significantly blocked any LTB4- or LTD4-stimulated TxA2 synthesis. Pretreatment with the TxA2 synthetase inhibitor ketoconazole (10(-6)M) also prevented LTB4 of LTD4 stimulation of TxA2. Preincubation with DMTU (10(-5)M), an hydroxyl radical scavenger, decreased LTB4-induced release of TxA2 (405 +/- 40 and 366 +/- 20, respectively). These findings suggest that LT may mediate their inflammatory actions in vascular beds by stimulation of Tx release from endothelial cells.
B Dunham; D Shepro; H B Hechtman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Inflammation     Volume:  8     ISSN:  0360-3997     ISO Abbreviation:  Inflammation     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1984-12-14     Completed Date:  1984-12-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600105     Medline TA:  Inflammation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  313-21     Citation Subset:  IM    
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MeSH Terms
Aorta / cytology*,  metabolism
Cells, Cultured
Chromones / pharmacology
Endothelium / metabolism
Ketoconazole / pharmacology
Leukotriene B4 / pharmacology*
Thromboxane B2 / biosynthesis*
Thromboxanes / biosynthesis*
Grant Support
Reg. No./Substance:
0/Chromones; 0/Thromboxanes; 40786-08-1/FPL 55712; 54397-85-2/Thromboxane B2; 65277-42-1/Ketoconazole; 71160-24-2/Leukotriene B4

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