| Leukotriene induction of TxB2 in cultured bovine aortic endothelial cells. | |
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MedLine Citation:
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PMID: 6092275 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The leukotrienes (LT) are potent mediators of inflammation, capable of inducing plasma leakage from postcapillary venules and vasoconstriction of terminal arterioles. Some microvascular effects may be attributable to LT stimulation of thromboxane (Tx) synthesis. Incubation of primary cultures of bovine aortic endothelial cells with buffer, LTB4 (10(-8) M) or LTD4 (10(-8)M), resulted in TxA2 production in pg/10(5) cells to the extent of: 67 +/- 20, 571 +/- 180, and 333 +/- 60 respectively, as measured by radioimmunoassay of the stable metabolite TxB2. Endothelial pretreatment with the LTD4 receptor antagonist FPL55712 (10(-5)M) significantly blocked any LTB4- or LTD4-stimulated TxA2 synthesis. Pretreatment with the TxA2 synthetase inhibitor ketoconazole (10(-6)M) also prevented LTB4 of LTD4 stimulation of TxA2. Preincubation with DMTU (10(-5)M), an hydroxyl radical scavenger, decreased LTB4-induced release of TxA2 (405 +/- 40 and 366 +/- 20, respectively). These findings suggest that LT may mediate their inflammatory actions in vascular beds by stimulation of Tx release from endothelial cells. |
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Authors:
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B Dunham; D Shepro; H B Hechtman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Inflammation Volume: 8 ISSN: 0360-3997 ISO Abbreviation: Inflammation Publication Date: 1984 Sep |
Date Detail:
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Created Date: 1984-12-14 Completed Date: 1984-12-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600105 Medline TA: Inflammation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 313-21 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / cytology*, metabolism Cattle Cells, Cultured Chromones / pharmacology Endothelium / metabolism Ketoconazole / pharmacology Leukotriene B4 / pharmacology* Thromboxane B2 / biosynthesis* Thromboxanes / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
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GM24891/GM/NIGMS NIH HHS; HL 16714-08/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chromones; 0/Thromboxanes; 40786-08-1/FPL 55712; 54397-85-2/Thromboxane B2; 65277-42-1/Ketoconazole; 71160-24-2/Leukotriene B4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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