Document Detail


Leukotriene B4 and 20-OH-LTB4 in purulent peritoneal exudates demonstrated by GC-MS.
MedLine Citation:
PMID:  3016754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Inflammatory peritoneal exudates from 11 patients with purulent peritonitis or non-perforative appendicitis were analyzed by gas chromatography mass spectrometry (GC-MS) for their content of 5S, 12R-dihydroxy-6,14-cis-8,10-trans-eicosatetraenoic acid (LTB4) and its omega-oxidized catabolite, 5S,12R,20-trihydroxy-6,8,10,14-eicosatetraenoic acid (20-OH-LTB4). Eleven samples of peritoneal exudates were examined. LTB4 and 20-OH-LTB4 were demonstrated only in the samples from patients with purulent peritonitis. LTB4 was detected in 4 samples, and 20-OH-LTB4 was detected in 6 samples by GC-MS with selected ion monitoring. In addition, a nearly full spectrum corresponding to that of synthetic 20-OH-LTB4 was obtained with 3 samples. Neither LTB4 nor 20-OH-LTB4 were detected in samples from patients with non-perforative appendicitis. LTB4 or 20-OH-LTB4 may be related to the pathophysiological mechanism of purulent inflammations.
Authors:
Y Kikawa; Y Shigematsu; M Sudo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and medicine     Volume:  23     ISSN:  0262-1746     ISO Abbreviation:  Prostaglandins Leukot Med     Publication Date:  1986 Jul 
Date Detail:
Created Date:  1986-09-25     Completed Date:  1986-09-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8206868     Medline TA:  Prostaglandins Leukot Med     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  85-94     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Appendicitis / metabolism
Ascitic Fluid / metabolism*
Gas Chromatography-Mass Spectrometry
Humans
Inflammation / metabolism
Leukotriene B4 / analogs & derivatives*,  analysis,  metabolism*
Peritonitis / metabolism
Chemical
Reg. No./Substance:
71160-24-2/Leukotriene B4; 79008-24-5/5,12,20-trihydroxy-6,8,10,14-eicosatetraenoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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