Document Detail

Leukocyte-endothelial adherence correlates with pancreatic nitric oxide production in early cerulein-induced pancreatitis in rats.
MedLine Citation:
PMID:  9744651     Owner:  NLM     Status:  MEDLINE    
The role of nitric oxide (NO) in microcirculation during the development of acute pancreatitis was not clear. An in vivo microscopic technique was used for evaluating leukocyte-endothelial adherence in the pancreatic microcirculation after induction (cerulein) of acute pancreatitis. Microdialysis was performed to detect pancreatic nitrate concentration (NO level) by high-performance liquid chromatography. Cerulein caused significantly reduced flow velocity in 1 h (31 %) and increased the number of sticking leukocytes in 2 h; both persisted for at least 3 h. Pancreatic NO level was found to be significantly elevated (2.5-fold) in 1 h and also persisted for 3 h. Both microcirculatory changes and NO elevation were significantly alleviated in cerulein-induced animals pretreated with NO synthase inhibitor (NG-nitro-L-arginine), indicating that elevation of NO could precede and account for a major portion of the observed microcirculatory changes. Furthermore, there was a strong positive correlation between numbers of adherent leukocytes and pancreatic NO level, suggesting that during the development of acute pancreatitis, NO could play an adverse role in microcirculation.
H M Chen; M H Shyr; Y T Lau; T L Hwang; M F Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Shock (Augusta, Ga.)     Volume:  10     ISSN:  1073-2322     ISO Abbreviation:  Shock     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-03     Completed Date:  1998-12-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421564     Medline TA:  Shock     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  218-22     Citation Subset:  IM    
Department of Surgery, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taipei, Taiwan.
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MeSH Terms
Caerulein / toxicity*
Endothelium, Vascular / metabolism*
Enzyme Inhibitors / pharmacology
Glycodeoxycholic Acid / pharmacology
Leukocytes / pathology
NG-Nitroarginine Methyl Ester / pharmacology
Nitrates / analysis,  metabolism
Nitric Oxide / metabolism*
Pancreas / blood supply
Pancreatitis / chemically induced,  metabolism*,  pathology*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Nitrates; 10102-43-9/Nitric Oxide; 17650-98-5/Caerulein; 360-65-6/Glycodeoxycholic Acid; 50903-99-6/NG-Nitroarginine Methyl Ester

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