| Leukocyte-endothelial adherence correlates with pancreatic nitric oxide production in early cerulein-induced pancreatitis in rats. | |
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MedLine Citation:
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PMID: 9744651 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The role of nitric oxide (NO) in microcirculation during the development of acute pancreatitis was not clear. An in vivo microscopic technique was used for evaluating leukocyte-endothelial adherence in the pancreatic microcirculation after induction (cerulein) of acute pancreatitis. Microdialysis was performed to detect pancreatic nitrate concentration (NO level) by high-performance liquid chromatography. Cerulein caused significantly reduced flow velocity in 1 h (31 %) and increased the number of sticking leukocytes in 2 h; both persisted for at least 3 h. Pancreatic NO level was found to be significantly elevated (2.5-fold) in 1 h and also persisted for 3 h. Both microcirculatory changes and NO elevation were significantly alleviated in cerulein-induced animals pretreated with NO synthase inhibitor (NG-nitro-L-arginine), indicating that elevation of NO could precede and account for a major portion of the observed microcirculatory changes. Furthermore, there was a strong positive correlation between numbers of adherent leukocytes and pancreatic NO level, suggesting that during the development of acute pancreatitis, NO could play an adverse role in microcirculation. |
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Authors:
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H M Chen; M H Shyr; Y T Lau; T L Hwang; M F Chen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: 10 ISSN: 1073-2322 ISO Abbreviation: Shock Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1998-12-03 Completed Date: 1998-12-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 218-22 Citation Subset: IM |
Affiliation:
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Department of Surgery, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taipei, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caerulein / toxicity* Endothelium, Vascular / metabolism* Enzyme Inhibitors / pharmacology Glycodeoxycholic Acid / pharmacology Leukocytes / pathology Male Microcirculation NG-Nitroarginine Methyl Ester / pharmacology Nitrates / analysis, metabolism Nitric Oxide / metabolism* Pancreas / blood supply Pancreatitis / chemically induced, metabolism*, pathology* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Nitrates; 10102-43-9/Nitric Oxide; 17650-98-5/Caerulein; 360-65-6/Glycodeoxycholic Acid; 50903-99-6/NG-Nitroarginine Methyl Ester |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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