Document Detail


Leukocyte elastase inhibitor: a new regulator of PARP-1.
MedLine Citation:
PMID:  19723034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Poly(ADP-ribose) polymerase-1 (PARP-1) uses NAD(+) as a substrate to form ADP-ribose. During apoptosis, caspases cleave PARP-1 to avoid excessive NAD consumption. Because PARP-1 is a key regulator of the activity of DNases involved in caspase-dependent apoptosis, its cleavage is required to promote DNA degradation. To explore the situation in caspase-independent cell death, we investigated the effect of PARP-1 on the acid endonuclease leukocyte elastase inhibitor (LEI)-derived DNase II (L-DNase II). We found for the first time an association between PARP-1 and LEI/L-DNase II. Unexpectedly, we observed that LEI influenced the automodification of PARP-1.
Authors:
Chloé Leprêtre; Giuseppina Sidoli; A Ivana Scovassi; Alicia Torriglia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1171     ISSN:  1749-6632     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-09-02     Completed Date:  2009-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  25-31     Citation Subset:  IM    
Affiliation:
Université Pierre et Marie Curie-Paris 6, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate Ribose / metabolism*
Animals
Blotting, Western
Cell Line
Cell Nucleus / drug effects,  metabolism
Cytoplasm / drug effects,  metabolism
Electrophoresis, Polyacrylamide Gel
Endodeoxyribonucleases / genetics,  metabolism*
Fluorescent Antibody Technique
Hydrogen Peroxide / pharmacology
Immunohistochemistry
Leukocyte Elastase / metabolism
NAD / metabolism
Poly(ADP-ribose) Polymerases / metabolism*
Recombinant Proteins / metabolism
Swine
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 20762-30-5/Adenosine Diphosphate Ribose; 53-84-9/NAD; 7722-84-1/Hydrogen Peroxide; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.22.1/deoxyribonuclease II; EC 3.4.21.37/Leukocyte Elastase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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