Document Detail


Leukocyte-derived growth factor links the PDGF and CXC chemokine families of peptides.
MedLine Citation:
PMID:  8836048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Leukocytes produce many biological mediators that orchestrate the subsequent cellular events during wound healing. We have identified a novel cytokine, leukocyte-derived growth factor (LDGF), which is mitogenic for connective tissue cells. Sequence analysis of the LDGF peptide revealed that it is a precursor of other known peptides including platelet basic protein (PBP), connective tissue activating peptide III (CTAP-III), and neutrophil activating peptide 2 (NAP-2). None of these shorter peptides are active as mitogens for fibroblasts. LDGF appears to stimulate fibroblast growth by stimulation of tyrosine kinase activity of the PDGF receptors. One of the truncated products of LDGF, NAP-2, is a potent neutrophil chemoattractant. Peptides larger than NAP-2, such as PBP and CTAP-III, are not active as neutrophil chemoattractants. Collectively, these findings demonstrate that the LDGF peptide must remain intact in order to retain its fibroblast mitogenic activity. If the LDGF peptide is processed to release the carboxyl terminal half to generate NAP-2, a peptide with proinflammatory activity is generated. These results indicate that the multiple peptides produced from the LDGF-PBP gene posses divergent biological activities that could regulate different phases of the repair process.
Authors:
N Iida; M Haisa; A Igarashi; D Pencev; G R Grotendorst
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  10     ISSN:  0892-6638     ISO Abbreviation:  FASEB J.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-11-14     Completed Date:  1996-11-14     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1336-45     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Anatomy, University of Miami School of Medicine, Florida 33101, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Chemokines / metabolism*
Growth Substances* / genetics,  isolation & purification,  metabolism
Humans
Leukocytes / metabolism*
Molecular Sequence Data
Platelet-Derived Growth Factor / metabolism*
Protein Precursors / genetics,  isolation & purification,  metabolism
Recombinant Proteins / metabolism
Sequence Alignment
Sequence Analysis
Chemical
Reg. No./Substance:
0/Chemokines; 0/Growth Substances; 0/Platelet-Derived Growth Factor; 0/Protein Precursors; 0/Recombinant Proteins

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