Document Detail


Leukocyte-depleted secondary blood cardioplegia attenuates reperfusion injury after myocardial ischemia.
MedLine Citation:
PMID:  14571340     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Activated neutrophils have been implicated in reperfusion injury of the myocardium; leukocyte depletion at the time of reperfusion may contribute to better myocardial protection after cardiac surgery. In the present study, we examined whether leukocyte depletion as an adjunct to terminal blood cardioplegia attenuates reperfusion injury. METHODS: Porcine hearts that had undergone 60 minutes of normothermic ischemia with cardioplegia and 60 minutes of reperfusion under cardiopulmonary bypass were divided into four groups according to the methods of 15 min of controlled initial reperfusion: whole blood reperfusion (n = 6), leukocyte-depleted reperfusion (n = 6), secondary blood cardioplegia (n = 6) and leukocyte-depleted secondary blood cardioplegia (n = 6). At 60 min of reperfusion, hemodynamic recovery, release of malondialdehyde (MDA) as a marker for free oxygen radicals, CK-MB-isoenzyme from the coronary sinus, recovery of adenosine triphosphate, and myocardial water content were evaluated. RESULTS: The group with leukocyte-depleted secondary blood cardioplegia showed the best hemodynamic recovery (Emax and total dp/dt), lowest levels of MDA, CK-MB and myocardial water content, and highest adenosine triphosphate recovery. CONCLUSIONS: These results suggest that controlled reperfusion with leukocyte-depleted secondary blood cardioplegia attenuated severe damage of the myocardium as compared to whole blood reperfusion.
Authors:
A Civelek; M Roth; P Lemke; W P Klövekorn; E P Bauer
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Thoracic and cardiovascular surgeon     Volume:  51     ISSN:  0171-6425     ISO Abbreviation:  Thorac Cardiovasc Surg     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-22     Completed Date:  2004-03-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7903387     Medline TA:  Thorac Cardiovasc Surg     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  249-54     Citation Subset:  IM    
Affiliation:
Department of Thoracic and Cardiovascular Surgery, Bad Nauheim, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Component Transfusion / methods
Blood Transfusion / methods*
Heart Arrest, Induced / methods*
Leukocyte Count
Myocardial Ischemia / complications*
Reperfusion Injury / immunology*,  therapy*
Swine

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