| Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy. | |
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MedLine Citation:
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PMID: 21228037 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Zebrafish were proposed as an alternative to mammalian models to assess the efficacy and toxicity of antileukemic drugs. Due to the limited number of transgenic zebrafish leukemia models, we explored human leukemic cell xenograft in zebrafish embryos. Human leukemic cell lines and blast cells sorted from patients with acute myelogenous leukemia were injected 48 hours post-fertilization and remained in the circulation of zebrafish embryos for several days without affecting their development. Imatinib and oxaphorines did not demonstrate any toxicity on normal zebrafish embryos and decreased the leukemic burden in animals xenografted with sensitive leukemic cell lines. Two other molecules, all-trans retinoic acid and the translation inhibitor 4EGI-1, demonstrated teratogenic effects at concentrations shown to be efficient in vitro, which precluded investigation of their antileukemic activity in such models. Altogether, xenografted leukemic cells in zebrafish embryos are a pharmacologically relevant model for screening non-teratogenic drugs. |
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Authors:
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Benoist Pruvot; Arnaud Jacquel; Nathalie Droin; Patrick Auberger; Didier Bouscary; Jérome Tamburini; Marc Muller; Michaela Fontenay; Johanna Chluba; Eric Solary |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-01-12 |
Journal Detail:
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Title: Haematologica Volume: 96 ISSN: 1592-8721 ISO Abbreviation: Haematologica Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-01 Completed Date: 2011-08-16 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 0417435 Medline TA: Haematologica Country: Italy |
Other Details:
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Languages: eng Pagination: 612-6 Citation Subset: IM |
Affiliation:
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Inserm UMR866, Faculty of Medicine, Dijon, France. eric.solary@igr.fr |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents / pharmacology* Cell Line, Tumor / drug effects Dose-Response Relationship, Drug Drug Evaluation, Preclinical* Humans Jurkat Cells K562 Cells Piperazines / pharmacology Pyrimidines / pharmacology Tretinoin / pharmacology Xenograft Model Antitumor Assays* Zebrafish / surgery* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Piperazines; 0/Pyrimidines; 302-79-4/Tretinoin; BKJ8M8G5HI/imatinib |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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