Document Detail


Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy.
MedLine Citation:
PMID:  21228037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Zebrafish were proposed as an alternative to mammalian models to assess the efficacy and toxicity of antileukemic drugs. Due to the limited number of transgenic zebrafish leukemia models, we explored human leukemic cell xenograft in zebrafish embryos. Human leukemic cell lines and blast cells sorted from patients with acute myelogenous leukemia were injected 48 hours post-fertilization and remained in the circulation of zebrafish embryos for several days without affecting their development. Imatinib and oxaphorines did not demonstrate any toxicity on normal zebrafish embryos and decreased the leukemic burden in animals xenografted with sensitive leukemic cell lines. Two other molecules, all-trans retinoic acid and the translation inhibitor 4EGI-1, demonstrated teratogenic effects at concentrations shown to be efficient in vitro, which precluded investigation of their antileukemic activity in such models. Altogether, xenografted leukemic cells in zebrafish embryos are a pharmacologically relevant model for screening non-teratogenic drugs.
Authors:
Benoist Pruvot; Arnaud Jacquel; Nathalie Droin; Patrick Auberger; Didier Bouscary; Jérome Tamburini; Marc Muller; Michaela Fontenay; Johanna Chluba; Eric Solary
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-01-12
Journal Detail:
Title:  Haematologica     Volume:  96     ISSN:  1592-8721     ISO Abbreviation:  Haematologica     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-01     Completed Date:  2011-08-16     Revised Date:  2013-07-02    
Medline Journal Info:
Nlm Unique ID:  0417435     Medline TA:  Haematologica     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  612-6     Citation Subset:  IM    
Affiliation:
Inserm UMR866, Faculty of Medicine, Dijon, France. eric.solary@igr.fr
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / pharmacology*
Cell Line, Tumor / drug effects
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical*
Humans
Jurkat Cells
K562 Cells
Piperazines / pharmacology
Pyrimidines / pharmacology
Tretinoin / pharmacology
Xenograft Model Antitumor Assays*
Zebrafish / surgery*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Piperazines; 0/Pyrimidines; 302-79-4/Tretinoin; BKJ8M8G5HI/imatinib
Comments/Corrections

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