Document Detail


Leukemia-related chromosomal loss detected in hematopoietic progenitor cells of benzene-exposed workers.
MedLine Citation:
PMID:  22643707     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Benzene exposure causes acute myeloid leukemia and hematotoxicity, shown as suppression of mature blood and myeloid progenitor cell numbers. As the leukemia-related aneuploidies monosomy 7 and trisomy 8 previously had been detected in the mature peripheral blood cells of exposed workers, we hypothesized that benzene could cause leukemia through the induction of these aneuploidies in hematopoietic stem and progenitor cells. We measured loss and gain of chromosomes 7 and 8 by fluorescence in situ hybridization in interphase colony-forming unit-granulocyte-macrophage (CFU-GM) cells cultured from otherwise healthy benzene-exposed (n=28) and unexposed (n=14) workers. CFU-GM monosomy 7 and 8 levels (but not trisomy) were significantly increased in subjects exposed to benzene overall, compared with levels in the control subjects (P=0.0055 and P=0.0034, respectively). Levels of monosomy 7 and 8 were significantly increased in subjects exposed to <10 p.p.m. (20%, P=0.0419 and 28%, P=0.0056, respectively) and ≥ 10 p.p.m. (48%, P=0.0045 and 32%, 0.0354) benzene, compared with controls, and significant exposure-response trends were detected (P(trend)=0.0033 and 0.0057). These data show that monosomies 7 and 8 are produced in a dose-dependent manner in the blood progenitor cells of workers exposed to benzene, and may be mechanistically relevant biomarkers of early effect for benzene and other leukemogens.
Authors:
L Zhang; Q Lan; Z Ji; G Li; M Shen; R Vermeulen; W Guo; A E Hubbard; C M McHale; S M Rappaport; R B Hayes; M S Linet; S Yin; M T Smith; N Rothman
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-05-30
Journal Detail:
Title:  Leukemia     Volume:  26     ISSN:  1476-5551     ISO Abbreviation:  Leukemia     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-12     Completed Date:  2013-02-12     Revised Date:  2013-04-16    
Medline Journal Info:
Nlm Unique ID:  8704895     Medline TA:  Leukemia     Country:  England    
Other Details:
Languages:  eng     Pagination:  2494-8     Citation Subset:  IM    
Affiliation:
School of Public Health, Division of Environmental Health Sciences, University of California, Berkeley, CA, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aneuploidy
Benzene / adverse effects*
Case-Control Studies
Chromosome Deletion*
Chromosomes, Human, Pair 7 / genetics*
Chromosomes, Human, Pair 8 / genetics*
Colony-Forming Units Assay
Cross-Sectional Studies
Female
Follow-Up Studies
Humans
In Situ Hybridization, Fluorescence
Leukemia, Myeloid, Acute / chemically induced*,  genetics,  pathology
Male
Monosomy*
Occupational Exposure / adverse effects*
Prognosis
Grant Support
ID/Acronym/Agency:
N01-CO-12400/CO/NCI NIH HHS; P30ES01896/ES/NIEHS NIH HHS; P30ES10126/ES/NIEHS NIH HHS; P42ES05948/ES/NIEHS NIH HHS; R01ES06721/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
71-43-2/Benzene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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