| Leukemia L1210 cell lines resistant to ribonucleotide reductase inhibitors. | |
| | |
MedLine Citation:
|
PMID: 3276399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Leukemia L1210 cell lines, ED1 and ED2, were generated which were resistant to the cytotoxic effects of deoxyadenosine/erythro-9-(2-hydroxyl-3-nonyl)adenine and deoxyadenosine/erythro-9-(2-hydroxyl-3-nonyl)adenine plus 2,3-dihydro-1H-pyrazole[2,3a]imidazole/Desferal, respectively. The ED1 and ED2 were characterized to show that these cell lines had increased levels of ribonucleotide reductase as measured by CDP reduction. The reductase activity in crude cell-free extracts from the ED1 and ED2 cells was not inhibited by dATP. For CDP reductase, the activation by adenylylimido diphosphate and inhibition by dGTP and dTTP in these extracts from the ED1 and ED2 cells were the same as for the wild-type L1210 cells. The ED1 and ED2 cells were highly cross-resistant, as measured by growth inhibition, to deoxyguanosine/8-aminoguanosine, 2-fluorodeoxyadenosine, and 2-fluoroadenine arabinoside. While the ED2 cells showed resistance to 2,3-dihydro-1H-pyrazole-[2,3a]-imidazole/Desferal (6-fold), the ED1 and ED2 cell lines showed less resistance to hydroxyurea, 4-methyl-5-amino-1-formylisoquinoline thiosemicarbazone, and the dialdehyde of inosine. These data indicate that the mechanisms of resistance to the ribonucleotide reductase inhibitors are related to the increased level of ribonucleotide reductase activity and to the decreased sensitivity of the effector-binding subunit to dATP. |
| | |
Authors:
|
J G Cory; G L Carter |
Related Documents
:
|
8646799 - Nucleotide excision repair in the human ovarian carcinoma cell line (2008) and its cisp... 10606239 - Camptothecin resistance: role of the atp-binding cassette (abc), mitoxantrone-resistanc... 15652229 - High effectiveness of platinum(iv) complex with adamantylamine in overcoming resistance... 19528459 - 14-3-3sigma-dependent resistance to cisplatin. 20154269 - Bcl2 and casp8 regulation by nf-kappab differentially affect mitochondrial function and... 21194729 - Cd1a immunopositivity in perivascular epithelioid cell neoplasms: true expression or te... 12420569 - A study on cryopreservation of cultured rabbit periodontal ligament cells. 22072329 - Contrasting dna damage checkpoint responses in epithelium of the human seminal vesicle ... 19725869 - Efficient and selective tumor cell lysis and induction of apoptosis in melanoma cells b... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Cancer research Volume: 48 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1988 Feb |
Date Detail:
|
Created Date: 1988-03-14 Completed Date: 1988-03-14 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 839-43 Citation Subset: IM |
Affiliation:
|
Department of Internal Medicine, University of South Florida College of Medicine, Tampa 33612. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Deaminase
/
metabolism Adenosine Kinase / metabolism Animals Antineoplastic Agents* Cell Division / drug effects Cell Line Kinetics Leukemia L1210 / drug therapy*, enzymology Mice Nucleosides / pharmacology, therapeutic use* Ribonucleotide Reductases / antagonists & inhibitors* Structure-Activity Relationship |
| Grant Support | |
ID/Acronym/Agency:
|
CA 27398/CA/NCI NIH HHS; CA 42070/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antineoplastic Agents; 0/Nucleosides; EC 1.17.4.-/Ribonucleotide Reductases; EC 2.7.1.20/Adenosine Kinase; EC 3.5.4.4/Adenosine Deaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Increased resistance to cis-diamminedichloroplatinum(II) in NIH 3T3 cells transformed by ras oncogen...
Next Document: Transforming growth factors produced by normal and neoplastically transformed rat liver epithelial c...