Document Detail

Leukaemic cell growth and aggregation in vitro.
MedLine Citation:
PMID:  2464507     Owner:  NLM     Status:  MEDLINE    
Experiments were performed with two lymphoblastic mouse leukaemia, L1210 VA II cell population growing in vitro for 90 and 400 days. These cells were cultured for 9 days in Eagle's solution without exchanging the medium, and each day a sample was taken for determination of its density, viability of cells and cell diameter. In the cells of the 400 day old population the presence and number of aggregates was checked. Lipid, nucleotide, nucleic acid, DNA and protein contents were determined in these cells. The present experiment in vitro was arranged to observe an aggregation of leukaemia cells during culture without change of the medium. The density and viability of cells changes of their metabolism in this system may stimulate the situation in vivo after dissemination of cells to a new place.
B Dominiak; B Barcew; J Kawiak
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  26     ISSN:  0239-8508     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  1988  
Date Detail:
Created Date:  1989-03-23     Completed Date:  1989-03-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  203-8     Citation Subset:  IM    
Department of Histology and Embryology, Pomeranian Academy of Medicine, Szczecin, Poland.
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MeSH Terms
Cell Aggregation
Cell Division
Cell Line
DNA / analysis
Leukemia, Lymphoid / pathology*
RNA / analysis
Tumor Cells, Cultured / analysis
Reg. No./Substance:
63231-63-0/RNA; 9007-49-2/DNA

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