Document Detail


Leukaemic B-cell maturation: studies on immunoglobulin secretion mediated by two signalling systems in vitro.
MedLine Citation:
PMID:  8681608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The in vitro production and secretion of the immunoglobulins IgM, IgG, Kappa and lambda light chains by peripheral blood lymphocytes from chronic lymphocytic leukaemia patients was examined. The ELISA system was employed to quantitate the immunoglobulins secreted in the culture supernatants. Lipopolysaccharide and phorbol esters with CA++ ionophore were used as lymphocyte stimulators. The maturation of cultured lymphocytes was demonstrated by synthesis of cytoplasmic immunoglobulins and Ig secretions typical for plasma cells. In one case an isotype switch C mu for C gamma was observed.
Authors:
H Antosz; D Koczkodaj; B Kwiatkowska-Drabik; W Wach
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytobios     Volume:  84     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  1995  
Date Detail:
Created Date:  1996-08-22     Completed Date:  1996-08-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  31-40     Citation Subset:  IM    
Affiliation:
Department of Human Genetics, Medical Academy Lublin, Poland.
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MeSH Terms
Descriptor/Qualifier:
B-Lymphocytes / cytology,  immunology*,  secretion
Cell Differentiation / immunology
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G / metabolism
Immunoglobulin M / metabolism
Immunoglobulin kappa-Chains / metabolism
Immunoglobulin lambda-Chains / metabolism
Immunoglobulins / biosynthesis,  metabolism*
Immunophenotyping
Leukemia, B-Cell
Leukemia, Lymphocytic, Chronic, B-Cell
Lipopolysaccharides / pharmacology
Lymphocyte Activation / drug effects
Signal Transduction / immunology*
Tetradecanoylphorbol Acetate / pharmacology
Tumor Cells, Cultured / cytology,  immunology
Chemical
Reg. No./Substance:
0/Immunoglobulin G; 0/Immunoglobulin M; 0/Immunoglobulin kappa-Chains; 0/Immunoglobulin lambda-Chains; 0/Immunoglobulins; 0/Lipopolysaccharides; 16561-29-8/Tetradecanoylphorbol Acetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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